Development and evaluation of a novel contamination device that targets multiple life-stages of Aedes aegypti
Tóm tắt
The increasing global threat of Dengue demands new and easily applicable vector control methods. Ovitraps provide a low-tech and inexpensive means to combat Dengue vectors. Here we describe the development and optimization process of a novel contamination device that targets multiple life-stages of the Aedes aegypti mosquito. Special focus is directed to the diverse array of control agents deployed in this trap, covering adulticidal, larvicidal and autodissemination impacts. Different trap prototypes and their parts are described, including a floater to contaminate alighting gravid mosquitoes. The attractiveness of the trap, different odor lures and floater design were studied using fluorescent powder adhering to mosquito legs and via choice tests. We demonstrate the mosquitocidal impacts of the control agents: a combination of the larvicide pyriproxyfen and the adulticidal fungus Beauveria bassiana. The impact of pyriproxyfen was determined in free-flight dissemination experiments. The effect on larval development inside the trap and in surrounding breeding sites was measured, as well as survival impacts on recaptured adults. The developmental process resulted in a design that consists of a black 3 Liter water-filled container with a ring-shaped floater supporting vertically placed gauze dusted with the control agents. On average, 90% of the mosquitoes in the fluorescence experiments made contact with the gauze on the floater. Studies on attractants indicated that a yeast-containing tablet was the most attractive odor lure. Furthermore, the fungus Beauveria bassiana was able to significantly increase mortality of the free-flying adults compared to controls. Dissemination of pyriproxyfen led to >90% larval mortality in alternative breeding sites and 100% larval mortality in the trap itself, against a control mortality of around 5%. This ovitrap is a promising new tool in the battle against Dengue. It has proven to be attractive to Aedes aegypti mosquitoes and effective in contaminating these with Beauveria bassiana. Furthermore, we show that the larvicide pyriproxyfen is successfully disseminated to breeding sites close to the trap. Its low production and operating costs enable large scale deployment in Dengue-affected locations.
Tài liệu tham khảo
Dengue and severe dengue, fact sheet N°117.http://www.who.int/mediacentre/factsheets/fs117/en/index.html,
Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, Moyes CL, Drake JM, Brownstein JS, Hoen AG, Sankoh O, Myers MF, George DB, Jaenisch T, Wint WGR, Simmons CP, Scott TW, Farrar JJ, Hay SI: The global distribution and burden of dengue. Nature. 2013, 496: 504-507. 10.1038/nature12060.
Harrington J, Kroeger A, Runge-Ranzinger S, O’Dempsey T: Detecting and responding to a dengue outbreak: evaluation of existing strategies in country outbreak response planning. J Trop Med. 2013, 2013: 756832-
Reiter P: Oviposition, dispersal, and survival in aedes aegypti: implications for the efficacy of control strategies. Vector Borne Zoonotic Dis. 2007, 7: 261-273. 10.1089/vbz.2006.0630.
Maciel-de-Freitas R, Lourenco-de-Oliveira R: Does targeting key-containers effectively reduce aedes aegypti population density?. Tropical Medicine Int Health: TM & IH. 2011, 16: 965-973. 10.1111/j.1365-3156.2011.02797.x.
Fay RW, Elison DA: A preferred oviposition site as a surveillance method for aedes aegypti. Mosq news. 1966, 26: 531-535.
Facchinelli L, Valerio L, Pombi M, Reiter P, Costantini C, Della Torre A: Development of a novel sticky trap for container-breeding mosquitoes and evaluation of its sampling properties to monitor urban populations of aedes albopictus. Med Vet Entomol. 2007, 21: 183-195. 10.1111/j.1365-2915.2007.00680.x.
Chadee DD, Ritchie SA: Efficacy of sticky and standard ovitraps for aedes aegypti in Trinidad, West Indies. J Vector Ecol: journal of the Society for Vector Ecology. 2010, 35: 395-400. 10.1111/j.1948-7134.2010.00098.x.
Santana AL, Roque RA, Eiras AE: Characteristics of grass infusions as oviposition attractants to aedes (Stegomyia) (Diptera: Culicidae). J Med Entomol. 2006, 43: 214-220. 10.1603/0022-2585(2006)043[0214:COGIAO]2.0.CO;2.
Ritchie SA: Effect of some animal feeds and oviposition substrates on aedes oviposition in ovitraps in Cairns, Australia. J Am Mosq Control Assoc. 2001, 17: 206-208.
Ponnusamy L, Xu N, Nojima S, Wesson DM, Schal C, Apperson CS: Identification of bacteria and bacteria-associated chemical cues that mediate oviposition site preferences by aedes aegypti. Proc Natl Acad Sci USA. 2008, 105: 9262-9267. 10.1073/pnas.0802505105.
Wong J, Stoddard ST, Astete H, Morrison AC, Scott TW: Oviposition site selection by the dengue vector aedes aegypti and its implications for dengue control. PLoS Negl Trop Dis. 2011, 5: e1015-10.1371/journal.pntd.0001015.
Eiras AE, Resende MC: Preliminary evaluation of the ‘dengue-MI’ technology for aedes aegypti monitoring and control. Cad Saude Publica. 2009, 25 (Suppl 1): S45-S58.
Resende MC, Azara TM, Costa IO, Heringer LC, Andrade MR, Acebal JL, Eiras AE: Field optimisation of MosquiTRAP sampling for monitoring aedes aegypti Linnaeus (Diptera: culicidae). Mem Inst Oswaldo Cruz. 2012, 107: 294-302. 10.1590/S0074-02762012000300002.
Gama RA, Silva EM, Silva IM, Resende MC, Eiras AE: Evaluation of the sticky MosquiTRAP for detecting aedes (stegomyia) aegypti (L.) (Diptera: culicidae) during the dry season in Belo Horizonte, Minas Gerais, Brazil. Neotrop Entomol. 2007, 36: 294-302. 10.1590/S1519-566X2007000200018.
de Santos EM, de Melo-Santos MA, de Oliveira CM, Correia JC, de Albuquerque CM: Evaluation of a sticky trap (AedesTraP), made from disposable plastic bottles, as a monitoring tool for aedes aegypti populations. Parasit Vectors. 2012, 5: 195-10.1186/1756-3305-5-195.
Ritchie SA, Long S, Hart A, Webb CE, Russell RC: An adulticidal sticky ovitrap for sampling container-breeding mosquitoes. J Am Mosq Control Assoc. 2003, 19: 235-242.
Williams CR, Ritchie SA, Long SA, Dennison N, Russell RC: Impact of a bifenthrin-treated lethal ovitrap on aedes aegypti oviposition and mortality in north Queensland, Australia. J Med Entomol. 2007, 44: 256-262. 10.1603/0022-2585(2007)44[256:IOABLO]2.0.CO;2.
Zeichner BC, Perich MJ: Laboratory testing of a lethal ovitrap for aedes aegypti. Med Vet Entomol. 1999, 13: 234-238. 10.1046/j.1365-2915.1999.00192.x.
Ritchie SA, Long SA, McCaffrey N, Key C, Lonergan G, Williams CR: A biodegradable lethal ovitrap for control of container-breeding aedes. J Am Mosq Control Assoc. 2008, 24: 47-53. 10.2987/5658.1.
Hemingway J, Ranson H: Insecticide resistance in insect vectors of human disease. Annu Rev Entomol. 2000, 45: 371-391. 10.1146/annurev.ento.45.1.371.
Caputo B, Ienco A, Cianci D, Pombi M, Petrarca V, Baseggio A, Devine GJ, Della Torre A: The “auto-dissemination” approach: a novel concept to fight aedes albopictus in urban areas. PLoS Negl Trop Dis. 2012, 6: e1793-10.1371/journal.pntd.0001793.
Itoh T, Kawada H, Abe A, Eshita Y, Rongsriyam Y, Igarashi A: Utilization of bloodfed females of aedes aegypti as a vehicle for the transfer of the insect growth regulator pyriproxyfen to larval habitats. J Am Mosq Control Assoc. 1994, 10: 344-347.
Ohba S-y, Ohashi K, Pujiyati E, Higa Y, Kawada H, Mito N, Takagi M: The effect of pyriproxyfen as a “population growth regulator” against aedes albopictus under semi-field conditions. PloS One. 2013, 8: e67045-10.1371/journal.pone.0067045.
Devine GJ, Perea EZ, Killeen GF, Stancil JD, Clark SJ, Morrison AC: Using adult mosquitoes to transfer insecticides to aedes aegypti larval habitats. Proc Natl Acad Sci U S A. 2009, 106: 11530-11534. 10.1073/pnas.0901369106.
Ponlawat A, Fansiri T, Kurusarttra S, Pongsiri A, McCardle PW, Evans BP, Richardson JH: Development and evaluation of a pyriproxyfen-treated device to control the dengue vector, aedes aegypti (L.) (Diptera:culicidae). Southeast Asian J Trop Med Public Health. 2013, 44: 167-178.
Farenhorst M, Mouatcho JC, Kikankie CK, Brooke BD, Hunt RH, Thomas MB, Koekemoer LL, Knols BG, Coetzee M: Fungal infection counters insecticide resistance in African malaria mosquitoes. Proc Natl Acad Sci U S A. 2009, 106: 17443-17447. 10.1073/pnas.0908530106.
Blanford S, Shi W, Christian R, Marden JH, Koekemoer LL, Brooke BD, Coetzee M, Read AF, Thomas MB: Lethal and pre-lethal effects of a fungal biopesticide contribute to substantial and rapid control of malaria vectors. PloS ONE. 2011, 6: e23591-10.1371/journal.pone.0023591.
Darbro JM, Johnson PH, Thomas MB, Ritchie SA, Kay BH, Ryan PA: Effects of beauveria bassiana on survival, blood-feeding success, and fecundity of aedes aegypti in laboratory and semi-field conditions. Am J Trop Med Hyg. 2012, 86: 656-664. 10.4269/ajtmh.2012.11-0455.
Dong Y, Morton JC, Ramirez JL, Souza-Neto JA, Dimopoulos G: The entomopathogenic fungus beauveria bassiana activate toll and JAK-STAT pathway-controlled effector genes and anti-dengue activity in aedes aegypti. Insect Biochem Mol Biol. 2012, 42: 126-132. 10.1016/j.ibmb.2011.11.005.
Kikankie CK, Brooke BD, Knols BG, Koekemoer LL, Farenhorst M, Hunt RH, Thomas MB, Coetzee M: The infectivity of the entomopathogenic fungus beauveria bassiana to insecticide-resistant and susceptible anopheles arabiensis mosquitoes at two different temperatures. Malar J. 2010, 9: 71-10.1186/1475-2875-9-71.
Farenhorst M, Knols BG, Thomas MB, Howard AF, Takken W, Rowland M, N’Guessan R: Synergy in efficacy of fungal entomopathogens and permethrin against west African insecticide-resistant anopheles gambiae mosquitoes. PloS One. 2010, 5: e12081-10.1371/journal.pone.0012081.
Ponnusamy L, Xu N, Boroczky K, Wesson DM, Abu Ayyash L, Schal C, Apperson CS: Oviposition responses of the mosquitoes aedes aegypti and aedes albopictus to experimental plant infusions in laboratory bioassays. J Chem Ecol. 2010, 36: 709-719. 10.1007/s10886-010-9806-2.
Bukhari T, Knols BG: Efficacy of aquatain, a monomolecular surface film, against the malaria vectors anopheles stephensi and an: gambiae s.s in the laboratory. Am J Trop Med Hyg. 2009, 80: 758-763.
Read AF, Lynch PA, Thomas MB: How to make evolution-proof insecticides for malaria control. PLoS Biol. 2009, 7: e1000058-
Barbosa RM, Souto A, Eiras AE, Regis L: Laboratory and field evaluation of an oviposition trap for culex quinquefasciatus (Diptera: culicidae). Mem Inst Oswaldo Cruz. 2007, 102: 523-529. 10.1590/S0074-02762007005000058.
Jackson BT, Paulson SL, Youngman RR, Scheffel SL, Hawkins B: Oviposition preferences of culex restuans and culex pipiens (Diptera: culicidae) for selected infusions in oviposition traps and gravid traps. J Am Mosq Control Assoc. 2005, 21: 360-365. 10.2987/8756-971X(2006)21[360:OPOCRA]2.0.CO;2.