Determination of total glycyrrhetic acid in Glycyrrhizae Radix by second derivative UV spectrometry
Tóm tắt
Second derivative (D2) spectrometry using ion-pair extraction technique was developed for the determination of total glycyrrhetic acid (GA) in Glycyrrhizae Radix. Glycyrrhizin (G) obtained from Glycyrrhizae Radix was hydrolyzed into GA in 2 N-HCl and methanol (1∶1) and extracted from aqueous phase in the form of an ion-pair complex with tetrapentylammonium bromide (TPA) as a counter ion. Maximum D2 amplitude (Z value) was obrained when 1000-fold or greater molar ratio of TPA was used at pH 11. Reaction time, temerature and ionic strength did not affect ion-pair formation. Dichloromethane was an effective extraction solvent of the ion-pair complex. The linearity of standard curve of ion-pair GA was obtained in the range of 4–120 μg/ml as GA. Assayed contents of GA in dry powder by D2 UV spectrometry and HPLC method were 5.31±0.04% and 5.20±0.008%, respectively.
Từ khóa
Tài liệu tham khảo
Kitamura, K., Takagi, M. and Hozumi, K.: Determination of aspirin and salicylic acid in aspirin tablets by second derivative ultraviolet spectrometry,Chem. Pharm. Bull. 32, 1484 (1984).
Kitamura, K. and Majima, R.: Determination of salicylic acid in aspirin power by second derivative ultraviolet spectrometry,Anal. Chem. 55, 54 (1983).
Such, V., Jordi, T. and Gonzalo, R.: Stability assays of aged pharmaceutucal formulas for thiamine and pyridoxine by high performance thin-layer chromatography and derivative ultraviolet spectrometry,Anal. Chem. 52, 412 (1980).
Ichikawa, T. and Terada, H.: Determination of phenylalanine, tryptophan and tyrosine in a mixture of amino acids by second derivative spectrophotometry,Chem. Pharm. Bull. 29, 438 (1981).
Mohamed, E.A., Magda, H.B., Ekram, M.H. and Mahamoud, A.E.: Spectrophotometric determination of ascorbic acid and thiamine hydrochloride in pharmaceutical products using derivative spectrophotometry,Analyst. 110, 813 (1985).
Lawrence, A.H. and Kovar, J.: Analysis of9-tetrahydrocannabinol-cannabinol, mixtures by second derivative ultraviolet spectrometry,Analyst. 110, 827 (1985).
Doyle, T.D. and Fazzari, F.R.: Determination of drugs in dosage forms by difference, spectrophotometry,Pharmaceutical Analysts.63, 1921 (1974).
Davodson, A.G. and Hassan, S.M.: Assay of benzenoid drugs in tablet and capsule formulations by second-derivative ultraviolet spectrophotometry,Journal of Pharmaceutical Sciences 73, 413 (1984).
Fell, A.F.: Biomedical applications of derivative spectroscopy,Trends in Analytical Chemistry 2, No. 3, (1983).
Kurono, G. and Sasaki, S.: Studies on the quantitative analysis of constituents in crude drugs by thin-layer chromatography. II. Studies on the quantitative analysis of glycyrrhizin in liquorice root by thin-layer chromatography.Yakugaku Zasshi,90, 497 (1970).
Pohl, P. and Hadrich, W.: Eine neue methods zur quantitativen bestimmung der glycyrrhinsaure in radix, liquiritiae.Deutsche Apotheker-Zeitung,116, 625 (1976).
Cundiff, R.H.: Spectrophotometric determination of glycyrrhizic acid in licorice extract.Analytical Chemistry,36, 1871 (1964).
Ichikawa, T., Ishida, S., Sakiya, Y. and Akada, Y.: High-performance liquid chromatographic determination of glycyrrhizin and glycyrrhetinic acid in biological materials.Chem. Pharm. Bull. 32, 3734 (1984).
Okada, K., Tanaka, J., Miyashita, A. and Imoto, K.: High-speed liquid chromatographic analysis of constituents in licorice root. I. Determination of glycyrrhizin.Yakugaku Zasshi,101, 822 (1982).
Akada, Y. and Tanase Y.: High-speed liquid chromatographic determination of glycyrrhizin in liquorice roots and extracts.Yakugaku Zasshi,96, 1035 (1976).
Higara, Y., Endo, H., Takahashi, K. and Shibata, S.: High-performance liquid chromatographic analysis of licorice extracts.J. Chromatography,292, 451 (1984).
Thomas, H.B., Howard, W.Z. and Alexis D.B.: Separation of major components in licorice using high-performance liquid chromatography.J. Chromatography,175, 350 (1979).
Sticher, V.O. and Soldan F.: Glycyrrhizin-saurebestimmung in radix liquiritiae mit hochlestungs-flussigkeitschromatographie (HPLC),Pharm. ACTAHELV.53, 46 (1978).
Sagara, K., Ito, Y., Oshima, T. and Misaki, T.: Application of ion-pair high-performance chromatography to the analysis of glycyrrhizin in Glycyrrhizae Radix.Chem. Pharm. Bull. 33, 5364 (1985).
Damon, L.: Quantitative determination of ammonium glycyrrhizinate by gas-liquid chromatography: Collaborative study.J. AOAC. 55, 570 (1972).
Kanaoka, M., Yano, S., Kato, H., Nakano, N. and Kinoshita, E.: Studies on the enzyme immunoasay of bio-active constituents contained in oriental medicinal drugs, II. Enzyme immunoassay of glycyrrhizin.Chem. Pharm. Bull. 31, 1866 (1983).
Kanaoka, M., Yano, S., Kato, H., Nakada, T. and Kawamura, K.: Stidies on the enzyme immunoassay of bio-active constituents contained in oriental medicinal drugs. IV. Enzyme immunoassay of glycyrrhetic acid.Chem. Pharm. Bull. 36, 8, (1988).