Determination of the survival of yeast and bacteria under the influence of gamma or UV radiation in the presence of some scavengers of OH radicals
Tóm tắt
Two new methods for quantitative determination of the role of scavengers of OH radicals in the radiation protection of various microorganisms were studied. The yeast and bacteria were irradiated with gamma or UVC radiations having quite different effect on living cells. Both methods based on the counting of colonies arising from surviving cells proved to be able to describe quantitatively the protective effect of scavengers. The described method may be helpful for study of the radiation protection of cells, radiation stability of DNA or the permeability of the cell membrane.
Tài liệu tham khảo
Múčka V, Bláha P, Čuba V, Červenák J (2013) Int J Radiat Biol 89:1045–1052
Copp RR, Peebles DD, Soref ChM, Fahl WE (2013) Int J Radiat Biol 89:485–492
Ewing D (1987) Application of radiation chemistry to studies in the radiation biology of microorganisms. In: Farhatazis, Rodgers MAJ (eds) Radiation chemistry. Principles and applications. WCH Publishers Inc, New York, pp 501–526
Von Sontag C (1987) The chemical bases of radiation biology, vol 3. Taylor and Francis Ltd (Inc), London, pp 97–104
Hirayama R, Ito A, Noguchi M, Matsumoto Y, Uzawa A, Kobashi G, Okayasu R, Furusawa Y (2013) Radiat Res 189:514–523
Hirayama R, Ito A, Tomita M, Tsukada T, Yatagai F, Noguchi M, Matsumoto Y, Kase Y, Ando K, Okayasu R, Furusawa Y (2009) Radiat Res 171:212–218
Roots R, Okada S (1972) Int J Radiat Biol 21:329–342
Olsson MG, Nilsson EJC, Rutardóttir S, Paczesuy J, Pallon J, Åkerström B (2010) Radiat Res 174:590–600
Buonanno M, de Toledo SM, Pain D, Azzam EI (2011) Radiat Res 175:405–415
Ito A, Nakano H, Kusano Y, Hirayama R, Furusawa Y, Murayama Ch, Mori T, Katsumura Y, Shinohara K (2006) Radiat Res 165:703–712
Lobachevsky PN, Vasireddy RJ, Broadhurst S, Sprung CN, Karagiannis TC, Smith AJ, Radford IR, Mc Kay MJ, Martin RF (2011) Int J Radiat Biol 87:274–283
Hou Y, Li X, Zhao Q, Chen G, Raston CL (2012) Environ Sci Technol 46:4042–4050
Mairami A, Böhlen TT, Dokic I, Cabal G, Brons S, Haberer T (2013) Int J Radiat Biol 89:782–793
Gomez FH, Loeschcke V, Norry M (2013) Int J Radiat Biol 89:583–589
Martignoni KD, Smith KC (1973) Photochem Photobiol 18:1–8
Remeikyté B, Pluklené R, Čitavičius D, Remeikis V, Vaitekonis Š (2004) Environ Chem Phys 26:148–156
Wu JY, Pan K, Ho TI (1998) In: Cooper WJ, Curry RD, O′Shea KE (eds) Environmental applications of ionizing radiation. John Wiley and Sons, INC, New York, p 291
Oppezzo OJ (2012) J Photochem Photobiol B Biol 115:58–62
Ahmad SI, Yokoi M, Hanaoka F (2012) J Photochem Photobiol B Biol 116:30–36
Wang W, Yu Y, An T, Li G, Yip HY, Yu JC, Wong PK (2012) Environ Sci Technol 46:4599–4606
Múčka V, Bláha P, Čuba V (2010) J Radioanal Nucl Chem 286:603–610
Mothersill C, Seymour C (2013) Radiat Environ Biophys 52:293–298
Fujii Y, Yurkon ChR, Maeda J, Genet SC, Okayasu R, Kitamura H, Fujimori A, Kato TA (2013) Int J Radiat Biol 89:401–410