Determination of fatty acid composition in seed oil of rapeseed (Brassica napus L.) by mutated alleles of the FAD3 desaturase genes
Tóm tắt
One of the goals in oilseed rape programs is to develop genotypes producing oil with low linolenic acid content (C18:3, ≤3%). Low linolenic mutant lines of canola rapeseed were obtained via chemical mutagenesis at the Plant Breeding and Acclimatization Institute – NRI, in Poznan, Poland, and allele-specific SNP markers were designed for monitoring of two statistically important single nucleotide polymorphisms detected by SNaPshot analysis in two FAD3 desaturase genes, BnaA.FAD3 and BnaC.FAD3, respectively. Strong negative correlation between the presence of mutant alleles of the genes and linolenic acid content was revealed by analysis of variance. In this paper we present detailed characteristics of the markers by estimation of the additive and dominance effects of the FAD3 genes with respect to particular fatty acid content in seed oil, as well as by calculation of the phenotypic variation of seed oil fatty acid composition accounted by particular allele-specific marker. The obtained percentage of variation in fatty acid composition was considerable only for linolenic acid content and equaled 35.6% for BnaA.FAD3 and 39.3% for BnaC.FAD3, whereas the total percentage of variation in linolenic acid content was 53.2% when accounted for mutations in both genes simultaneously. Our results revealed high specificity of the markers for effective monitoring of the wild-type and mutated alleles of the Brassica napus FAD3 desaturase genes in the low linolenic mutant recombinants in breeding programs.
Tài liệu tham khảo
Barret P, Delourme R, Brunel D, Jourdren C, Horvais R, Renard M (1999) Low linolenic acid level in rapeseed can be easily assesed through the detection of two single base substitution in FAD3 genes. Proc. 10th International Rapeseed Congress, Canberra, Australia, 26–29.09.1999. [CD-ROM]. Available at www.regional.org.au/au/gcirc/4/385.htm
Bocianowski J, Krajewski P (2009) Comparison of the genetic additive effect estimators based on phenotypic observations and on molecular marker data. Euphytica 165:113–122
Byczynska B, Krzymanski J (1969) A fast method for obtaining of fatty acids methyl esters to be analysed by gas chromatography method (in Polish). Tluszcze Jadalne XIII:108–114
Falconer DS, Mackay TFC (1996) Introduction to quantitative genetics. Addison-Wesley, Essex, pp 26–57
Hu X, Sullivan ML, Gupta M, Thompson SA (2006) Mapping of the loci controlling oleic and linolenic acid contents and development of fad2 and fad3 allele-specific markers in canola (Brassica napus L.). Theor Appl Genet 113:497–507
Jourdren C, Barret P, Horvais R, Delourme R, Renard M (1996) Identification of RAPD markers linked to linolenic acid genes in rapeseed. Euphytica 90:351–357
Mikolajczyk K, Dabert M, Karlowski WM, Spasibionek S, Nowakowska J, Cegielska-Taras T, Bartkowiak-Broda I (2010) Allele-specific SNP markers for the low linolenic mutant genotype of winter oilseed rape. Plant Breeding 129:502–507
Østergaard L, King GJ (2008) Standarized gene nomenclature for the Brassica genus. Plant Methods 4:10
Payne R, Murrey D, Harding S, Baird D, Soutou D, Lane P (2003) GenStat for Windows (7th edition) – Introduction. VSN International, Oxford, England
Spasibionek S (2006) New mutants of winter rapeseed (Brassica napus L.) with changed fatty acid composition. Plant Breeding 125:259–267