Determination of ampicillin and cloxacillin mixture by NMR
Tóm tắt
A simple, accurate and specific NMR procedure is described for the determination of ampicillin and cloxacillin mixtures in injection dosage form and capsules. The solvent was dimethylsulfoxde-d6 and maleic acid was the internal standard. By integrating the peak at 2.68 ppm and 4.57 ppm, cloxacillin and ampicillin could be determined respectively. The relative proton ratio of ampicillin trihydrate and cloxacillin were 1.038 and 0.950. The coefficents of variation of ampicillin trihydrate and cloxacillin in a few commercial preparation were 1.55% (n=9), 2.69% (n=15).
Tài liệu tham khảo
Wilsion, W. L., Avodovich, H. W., and Hughes, D. W., Applications of nuclear magnetic resonance spectroscopy to antibiotics. I. Specific identification of penicillins and cephalosporins.J. AOAC.,57, 1300 (1974).
Acred, P., and Sutherland, R., Antibacterial activities of combinations of ampicillin and cloxacillin.Antimicrob. Ag. Chemother.,53 (1966).
Sutherland, R., and Batchelor, F. R., Synergistic activity of penicillins against penicillinase-producing gram-negative bacteria.Nature,201, 868 (1964).
Saccani, F., Neri, C., and Sudano, F., Separation and determination of ampicillin from mixture with dicloxacillin or cloxacillin.Bull. Chim. Farm.,108, 777 (1969).
Murakawa, T., Wakai, Y., Nishida, M., Fujii, R., Konno M., Okada K., Cioto S., and Kuwahara S., Chromatographic assay of the mixed penicillins, ampicillin and cloxacillin in body fluids.J. Antibiot.,23, 250 (1970).
Celletti, P., Moretti, G. P., and Petrangeli B., New methods for chemical determination of ampicillin and cloxacillin alone or in combination.Farmaco. Ed. Prat.,27, 688 (1972).
Davidson, A. G., and Stenlake, J. B., The spectophotometric determination of ampicillin and cloxacillin in combined preparations.J. Pharm. Pharmacol.,25, (suppl.), 156 (1973).
De Leo, S., and Pitrolo, G., Iodometric determination of ampicillin and cloxacillin in a mixture.Bull. Chim. Farm.,112, 487 (1973).