Determination of Polyamines in Beers by HPTLC with Fluorescence Detection
Tóm tắt
A high-performance thin-layer chromatographic (HPTLC) method has been developed for determination of polyamines in beers. Beer samples, previously degassed, were derivatized with dansyl chloride and the dansyl derivatives were separated by HPTLC with silica gel as stationary phase and chloroform-triethylamine, 2 + 1 (v/v), containing 5% (m/v) polyoxyethylene-10-lauryl ether, as mobile phase. Quantitative analysis was achieved by in-situ fiberoptic-based fluorescence scanning. The compounds were determined over the range 0.5–85 ng, with relative standard deviations between 0.44–1.16% and detection limits in the range 0.28–0.39 ng. The simple preparation of the sample and the rapid microwave-assisted dansylation considerably reduce analysis time and effort.
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