Determination of HCV Genotype Using Two Antibody Assays and Genome Typing
Tóm tắt
Two serotyping assays for hepatitis C virus (Serotyping 1–6 assay; Murex, UK and RIBA Serotyping SI; Chiron, USA) were
compared to a standardized genotyping assay (Inno-LiPA HCV II;
Innogenetics, Belgium) using serum samples collected from 126 patients
chronically infected with hepatitis C virus. Serotyping was positive in
87% and 80% of the samples tested with Murex and Chiron, respectively,
and concordant with genotyping in 93% and 88%, respectively. Sequence
analysis of the NS5b region of 15 samples with discrepant typing results
confirmed the Inno-LiPA finding in all instances. The Murex enzyme immunoassay
serotyping method was less sensitive for identifying genotype 2 (P<0.05).
The concordance with genotyping of the RIBA serotyping
method was lower for genotype 2 than for the other genotypes (P<0.05).