Determination of C-terminal sequences by digestion with serine carboxypeptidases: The influence of enzyme specificity

Klaus Breddam1, Martin Ottesen1
1Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500, Copenhagen Valby

Tóm tắt

Từ khóa


Tài liệu tham khảo

Ambler, R.P.: Carboxypeptidases A and B. Eds. C. H. W. Hirs & S. n. Timasheff, Academic Press. Methods Enzymol. 25, 262–272 (1972)

Breddam, K., F. Widmer &J. T. Johansen: Carboxypeptidase Y catalyzed transpeptidations and enzymatic peptide synthesis. Carlsberg Res. Commun. 45, 237–247 (1980)

Breddam, K.: Modification of the single sulfhydryl group of carboxypeptidase Y. Carlsberg Res. Commun. 48, 9–19 (1983)

Breddam, K., S. B. Sørensen &M. Ottesen: Isolation of a carboxypeptidase from malted barley by affinity chromatography. Carlsberg Res. Commun. 48, 217–230 (1983)

Breddam, K. &M. Ottesen: Influence of guanidine derivatives on the specificity of malt carboxypeptidase. Carlsberg Res. Commun. 49, 573–582 (1983)

Breddam, K.: Chemically modified carboxypeptidase Y with increased amidase activity. Carlsberg Res. Commun. 49, 535–554 (1984)

Breddam, K., S. B. Sørensen &M. Ottesen: Isolation of carboxypeptidase II from malted barley by affinity chromatography. Carlsberg Res. Commun. 50, 199–209 (1985)

Breddam, K.: Enzymatic properties of malt carboxypeptidase II in hydrolysis and aminolysis reactions. Carlsberg Res. Commun. 50, 309–323 (1985)

Breddam, K.: Serine carboxypeptidases. A Review. Carlsberg Res. Commun. 51, 83–128 (1986)

Hayashi, R., Y. Bai &T. Hata: Kinetic studies of carboxypeptidase Y. I. Kinetic parameters for the hydrolysis of synthetic substrates. J. Biochem. (Tokyo) 77, 69–79 (1975)

Hayashi, R.: Carboxypeptidase Y in sequence determination of peptides. Eds. C. H. W. Hirs & S. N. Timasheff, Academic Press. Methods Enzymol. 47, 84–93 (1977)

Hirs, C. H. W.: Determination of cystine as cysteic acid. Meth. Enzymol. 45, 587–599 (1979)

Martin, B., I. Svendsen &M. Ottesen: Use of carboxypeptidase Y for carboxy terminal sequence determinations. Carlsberg Res. Commun. 42, 99–102 (1977)

Mikola, L. &J. Mikola: Mobilization of proline in the starchy endosperm of germinating barley grain. Planta 149, 149–154 (1980)

Schechter, I. &B. Berger: On the size of the active site of proteases. I. Papain. Biochem. Biophys. Res. Commun. 27, 157–162 (1967)

Tschesche, H.: Carboxypeptidase C. Eds. C. H. W. Hirs & S. N. Timasheff, Academic Press. Methods Enzymol. 47, 73–84 (1977)

Wagner, R. M.: Exopeptidase-high-performance liquid chromatography peptide mapping of small peptides. J. Chromatography. 326, 399–405 (1985)