Detection of nitric oxide in tissue by spin trapping EPR spectroscopy and triacetylglycerol extraction

Biotechnology Techniques - Tập 13 - Trang 507-511 - 1999
Guangyin Zhou1, Baolu Zhao1, Jingwu Hou1, Meifen Li1, Chang Chen1, Wenjuan Xin1
1Institute of Biophysics, Academia Sinica, Beijing, China

Tóm tắt

A modified method based on EPR spin trapping and triacetylglycerol extraction was used for tissue nitric oxide (NO) detection at room temperature. NO signal intensity was stable for about 1.5 h and the detection limit of this method was less than 200 pmol g−1 tissue. Using this method, we report evidence that NO production in vivo can be inhibited by adriamycin in mice livers.

Tài liệu tham khảo

Archer S (1993) Measurement of nitric oxide in biological models. FASEB J. 7: 349-360. Douki T, Cadet J (1996) Peroxynitrite mediated oxidation of purine bases of nucleosides and isolated DNA. Free Rad. Res. 24: 369-380. Ignarro LJ, Buga GM, Wood KS, Byrns RE, Chaudhuri G (1987) Endothelium-derived relaxing factor produced and released from artery and vein is nitric oxide. Proc. Natl. Acad. Sci. USA 84: 9265-9269. Ignarro LJ (1989) Endothelium-derived nitric oxide: pharmacology and relationship to the actions of organic nitrate esters. Pharmacol. Res. 6: 651-659. Kubrina LN, Caldwell WS, Mordvintcev PI, Malenkova IV, Vanin AF (1992) EPR evidence for nitric oxide production from guanidino nitrogens of L-arginine in animal tissues in vivo. Biochim. Biophys. Acta. 1099: 233-237. Luo DS, Vincent SR (1994) Inhibition of nitric oxide synthase by antineoplastic anthracyclines. Biochem. Pharmacol. 47: 2111-2112. Palmer RM, Ferrige AG, Moncada S (1987) Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor. Nature 327: 524-526. Tominaga T, Sato S, Ohnishi T, Ohnishi ST (1993) Potentiation of nitric oxide formation following bilateral carotid occlusion and focal cerebral ischemia in the rat: in vivo detection of the nitric oxide radical by electron paramagnetic resonance spin trapping. Brain Res. 614: 342-346. Tsuchiya K, Takasugi M, Minakuchi K, Fukuzawa K (1996) Sensitive quantitation of nitric oxide by EPR spectroscopy. Free Rad. Biol. Med. 21: 733-737. VasquezVivar J, Martasek N, Hogg N, Masters BSS, Pritchard KA, Kalyanaraman B (1997) Endothelial nitric oxide synthasedependent superoxide generation from adriamycin. Biochemistry 36: 11293-11297. Voevodskaya LN, Vanin, AF (1992) Gamma-irradiation potentiates L-arginine-dependent nitric oxide formation in mice. Biochem. Biophys. Res. Commun. 186: 1423-1428.