Detection of endogenous and food-derived collagen dipeptide prolylhydroxyproline (Pro-Hyp) in allergic contact dermatitis-affected mouse ear

Bioscience, Biotechnology and Biochemistry - Tập 79 Số 8 - Trang 1356-1361 - 2015
Masashi Kusubata1, Yoh‐ichi Koyama1, Chisa Tometsuka1, Yasutaka Shigemura2,3, Kenji Sato4,2
1Research Institute of Biomatrix, Nippi Inc., Toride, Japan
2Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, Japan
3Faculty of Domestic Science, Department of Nutrition, Tokyo Kasei University, Tokyo, Japan
4Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan

Tóm tắt

Abstract Generation of collagen dipeptides and deposition of orally administered prolylhydroxyproline (Pro-Hyp) in local inflammatory sites were examined in mice with hapten (2,4-dinitrofluorobenzene)-induced dermatitis in the ear. Pro-Hyp content in the hapten-treated ear was significantly higher in the chronic phase of contact dermatitis than the vehicle control. In contrast, hydroxyprolylglycine contents remained at lower levels in all cases compared to Pro-Hyp. Four hours after the ingestion of [13C5,15N]Pro and [13C5,15N]Pro-Hyp, labeled-Pro-Hyp and Pro, respectively, appeared only in the ear with dermatitis. Thus, Pro-Hyp is generated and degraded as part of the rapid synthesis and degradation of collagen in the ear with dermatitis. In addition to the endogenously generated Pro-Hyp, the orally administered Pro-Hyp was deposited in the ears.

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