Detection of Surfactant Proteins A, B, C, and D in Human Nasal Mucosa and Their Regulation in Chronic Rhinosinusitis with Polyps

American Journal of Rhinology and Allergy - Tập 27 Số 1 - Trang 24-29 - 2013
Martin Schicht1, Stephan Knipping2, Robert P. Hirt2, Stephanie Beileke3, Saadettin Sel4, Friedrich Paulsen1, Lars Bräuer1
1Department of Anatomy II, University of Erlangen-Nürnberg, Germany
2Department of Otorhinolaryngology, Head and Neck Surgery, Dessau Medical Center, Germany
3Department of Anatomy and Cell Biology, Martin-Luther-University of Halle-Wittenberg, Germany
4Department of Ophthalmology, University of Erlangen-Nürnberg, Germany

Tóm tắt

Backround The nasal mucosa is characterized by a multirow high prismatic ciliated epithelium representing the first barrier of the immune defense system against microbial and other environmental pathogenic influences. A number of nonspecific defense mechanisms, including the presence of lactoferrin, peroxidases, proteases, interferons, and lysozymes in nasal secretions, act to counter inflammatory processes. The surfactant proteins (SPs) known from the lungs are important components of the innate immune system. They also influence the rheology of fluids and reduce the surface tension of gas–fluid interphases. The objective of this study was to investigate the protein expression of all four SPs. A specific aim was detection and characterization of SP-C, which had previously not been confirmed in human nasal mucosa. Methods The expression of mRNA for SP-A, -B, -C and -D was investigated using reverse transcriptase polymerase chain reaction on samples of both healthy nasal mucosa and nasal mucosa altered by inflammatory processes (allergic rhinitis and chronic rhinosinusitis). The distribution of all four proteins was determined with monoclonal antibodies using Western blot analysis as well as immunohistochemical methods. Results The results show that all four SPs, including SP-C not detected before this, are nasal mucosa components. A shift was also observed in the expression behavior of the SP-A, -B, and -D in nasal mucosa with inflammatory changes. Conclusion Based on these results, SPs appear to have an important function in immunologic and rheological process of the nasal mucosa and support the prospective therapeutic use of liposomal nasal sprays.

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American Journal of Rhinology and Allergy

Tập 27 Số 1

24-29

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