Detection and widespread distribution of sodium channel alleles characteristic of insecticide resistance in Culex pipiens complex mosquitoes in China

Medical and Veterinary Entomology - Tập 26 Số 2 - Trang 228-232 - 2012
Zhongming Wang1, Chunxiao Li1, Dan Xing1, Yang Yu1, N. Liu2, Rui‐De Xue3, Yicheng Dong1, Tongyan Zhao1
1State Key Laboratory of Pathogen and Biosecurity, Department of Vector Biology and Control, Beijing Institute of Microbiology and Epidemiology, Beijing, P. R. China.
2Entomology and Plant Pathology, Auburn University, Auburn, AL, U.S.A.
3Anastasia Mosquito Control District, St. Augustine, FL, U.S.A.

Tóm tắt

Culex pipiens complex mosquitoes are widely distributed throughout China and are known to be important disease vectors. Two pyrethroid resistance associated mutations have been identified in Cx. pipiens complex (Diptera: Culicidae), but there is little information on the diversity and distribution of kdr alleles in pyrethroid resistance in Cx. pipiens complex mosquitoes in China. In the present study, we report on a modified three tube allele‐specific (AS)‐PCR method for detecting the 1014F and 1014S alleles. The new technique was applied to identify the distribution of the two alleles in natural Cx. pipiens complex populations in China. The results confirmed that the new method is both sensitive and specific. The 1014F allele was found in all 14 of the field populations tested (frequency ranged from 6.8 to 76.2%) and the 1014S allele was found in almost two‐thirds (frequency from 2.4 to 28.6%), indicating that the genotypes known to be associated with pyrethroid resistance are widespread in China. The resistance‐associated alleles were more common in southern Chinese sampling sites than in northern sites. The coexistence of the two resistant mutations in individual mosquitoes was also observed in five of the field populations. Two alternative mutations within the L1014 codon were identified in Culex pipiens molestus Forskal, 1775, including a non‐synonymous mutation resulting in a 1014C substitution.

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Thông tin
Thông tin xuất bản

Medical and Veterinary Entomology

Tập 26 Số 2

228-232

Thông tin tác giả