Detection and characterization of Streptomyces causing potato common scab in Western Europe

Plant Pathology - Tập 57 Số 1 - Trang 162-169 - 2008
R. Flores‐González1, Iván Marcelo Poveda Velasco1, Fátima Cristina Onofre Fandinho Montes1
1Laboratorio de Producción y Sanidad Vegetal de Sevilla, Apdo. 121, 41089 Montequinto, Sevilla, Spain

Tóm tắt

A PCR‐based diagnostic method was developed for direct detection from tuber lesions of pathogenic Streptomyces causing common scab of potato. Primers were designed to amplify a fragment of the txtAB (txtA and txtB) genes, which are pathogenicity determinants in the main pathogenic Streptomyces species. The method was evaluated on 84 naturally infected potato samples, comprising 19 potato cultivars, harvested in the years from 2000 to 2004 in the Netherlands, the UK, France, Germany and Spain. Pathogenic Streptomyces in tuber lesions were detected by PCR in 70 samples and were also successfully isolated from these 70 samples. All pathogenic isolates showed the basic general phenotypic traits of the S. scabiei phenetic cluster. RFLP analysis of amplified rRNA sequences, together with carbon source utilization and repetitive BOX profiles, allowed most isolates to be assigned to S. europaeiscabiei, which emerged as the main cause of potato common scab in Western Europe.

Từ khóa


Tài liệu tham khảo

10.1099/00207713-50-1-91

10.1128/jb.179.24.7776-7783.1997

10.1094/MPMI.1998.11.10.960

10.1128/AEM.68.2.738-744.2002

10.1094/PHYTO.1998.88.5.442

10.1099/00207713-41-4-479

Healy FG, 1997, Identification of thaxtomin A non‐producing mutants of Streptomyces scabies, Phytopathology, 87, S41

Healy FG, 1999, Characterization of an insertion sequence element associated with genetically diverse plant pathogenic Streptomyces spp, Journal of Bacteriology, 181, 1562, 10.1128/JB.181.5.1562-1568.1999

10.1046/j.1365-2958.2000.02170.x

10.1128/JB.184.7.2019-2029.2002

10.1111/j.1365-2958.2004.04461.x

10.1007/BF02853743

10.1021/jf00017a025

10.1094/PHYTO.1999.89.6.462

10.1099/00207713-39-4-387

10.1099/00207713-39-4-393

10.1094/Phyto-80-606

10.1094/Phyto-86-709

10.1016/S0167-7012(99)00033-0

10.1094/Phyto-85-537

10.1094/PDIS.1997.81.8.836

Loria R, 1999, Laboratory Guide for Identification of Plant Pathogenic Bacteria, 236

10.1146/annurev.phyto.44.032905.091147

10.1099/00207713-48-2-495

10.1094/PDIS.2003.87.11.1290

10.1099/ijs.0.02629-0

Sadowsky MJ, 1996, Use of repetitive intergenic DNA sequences to classify pathogenic and disease‐suppressive Streptomyces strains, Applied and Environmental Microbiology, 62, 3489, 10.1128/aem.62.9.3489-3493.1996

10.1099/00207713-16-3-313

10.1099/ijs.0.02624-0

10.1099/00207713-46-2-476

Versalovic J, 1994, Genomic fingerprinting of bacteria using repetitive sequence‐based polymerase chain reaction, Methods in Molecular and Cellular Biology, 5, 25

10.1093/nar/gkg526

10.1094/PDIS.2004.88.8.785

10.1094/PHYTO-96-1363

Thông tin
Thông tin xuất bản

Plant Pathology

Tập 57 Số 1

162-169

Thông tin tác giả