Detecting peroxiredoxin hyperoxidation by one-dimensional isoelectric focusing

Biophysics Reports - Tập 1 Số 1 - Trang 14-17 - 2015
Zhenbo Cao1, Neil J. Bulleid1
1Institute of Molecular, Cellular and Systems Biology, College of Medical Veterinary and Life Sciences, Davidson Building, University of Glasgow, Glasgow G12 8QQ, UK

Tóm tắt

Từ khóa


Tài liệu tham khảo

Cao Z, Subramaniam S, Bulleid NJ (2014) Lack of an efficient endoplasmic reticulum-localized recycling system protects peroxiredoxin iv from hyperoxidation. J Biol Chem 289:5490–5498

Cox AG, Winterbourn CC, Hampton MB (2010) Measuring the redox state of cellular peroxiredoxins by immunoblotting. Methods Enzymol 474:51–66

Day AM, Brown JD, Taylor SR, Rand JD, Morgan BA, Veal EA (2012) Inactivation of a peroxiredoxin by hydrogen peroxide is critical for thioredoxin-mediated repair of oxidized proteins and cell survival. Mol Cell 45:398–408

Edgar RS, Green EW, Zhao YW, van Ooijen G, Olmedo M, Qin XM, Xu Y, Pan M, Valekunja UK, Feeney KA, Maywood ES, Hastings MH, Baliga NS, Merrow M, Millar AJ, Johnson CH, Kyriacou CP, O’Neill JS, Reddy AB (2012) Peroxiredoxins are conserved markers of circadian rhythms (vol 485, pg 459, 2012). Nature 489:590

Turner-Ivey B, Manevich Y, Schulte J, Kistner-Griffin E, Jezierska-Drutel A, Liu Y, Neumann CA (2013) Role for Prdx1 as a specific sensor in redox-regulated senescence in breast cancer. Oncogene 32:5302–5314

Woo HA, Kang SW, Kim HK, Yang KS, Chae HZ, Rhee SG (2003) Reversible oxidation of the active site cysteine of peroxiredoxins to cysteine sulfinic acid—Immunoblot detection with antibodies specific for the hyperoxidized cysteine-containing sequence. J Biol Chem 278:47361–47364

Wood ZA, Poole LB, Karplus PA (2003a) Peroxiredoxin evolution and the regulation of hydrogen peroxide signaling. Science 300:650–653

Wood ZA, Schroder E, Harris JR, Poole LB (2003b) Structure, mechanism and regulation of peroxiredoxins. Trends Biochem Sci 28:32–40

Yang KS, Kang SW, Woo HA, Hwang SC, Chae HZ, Kim K, Rhee SG (2002) Inactivation of human peroxiredoxin I during catalysis as the result of the oxidation of the catalytic site cysteine to cysteine-sulfinic acid. J Biol Chem 277:38029–38036