Desulfonatronobacter acidivorans gen. nov., sp. nov. and Desulfobulbus alkaliphilus sp. nov., haloalkaliphilic heterotrophic sulfate-reducing bacteria from soda lakes

International Journal of Systematic and Evolutionary Microbiology - Tập 62 Số Pt_9 - Trang 2107-2113 - 2012
Dimitry Y. Sorokin1,2, T. P. Tourova2, A. N. Panteleeva3, Gerard Muyzer1
1Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands
2Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/2, 117312 Moscow, Russia
3Bioengineering Centre, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/1, 117811 Moscow, Russia

Tóm tắt

Two types of heterotrophic sulfate-reducing bacteria (SRB) were isolated from anoxic sediments of hypersaline soda lakes in Kulunda Steppe (Altai, Russia). The isolates used propionate as an energy and carbon source. Strain APT2Twas enriched and isolated with thiosulfate as the electron acceptor. Strains APS1Tand ASS1 were isolated with sulfate. Strain APT2Twas a short rod and motile with a single subpolar flagellum, while strains APS1Tand ASS1 were lemon-shaped oval rods and motile with a single polar flagellum and thin flagella-like filaments. Strain APT2Tgrew by complete oxidation of C3–C8fatty acids with thiosulfate or sulfate as the electron acceptor, while strains APS1Tand ASS1 were much less versatile and utilized only propionate and pyruvate as the electron donor and carbon source with sulfate or sulfite as the electron acceptor. Furthermore, strains APS1Tand ASS1 oxidized propionate incompletely to form acetate. All of the isolates were moderately halophilic and obligately alkaliphilic. Phylogenetic analysis based on 16S rRNA gene sequences placed the isolates in the orderDesulfobacteralesof the classDeltaproteobacteria. Strain APT2Tbelonged to the familyDesulfobacteraceaeand clustered with a halophilic SRB,Desulfosalsimonas propionicicaPropAT. Strains APS1Tand ASS1 were closely related to each other and clustered with the genusDesulfobulbusof the familyDesulfobulbaceae. On the basis of phenotypic and phylogenetic analysis, the isolates are proposed to represent two novel taxa,Desulfonatronobacter acidivoransgen. nov., sp. nov. (type strain of the type species APT2T = DSM 24257T = UNIQEM U853T) andDesulfobulbus alkaliphilussp. nov. (type strain APS1T = DSM 24258T = UNIQEM U900T).

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International Journal of Systematic and Evolutionary Microbiology

Tập 62 Số Pt_9

2107-2113

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