Designing a conserved peptide-based subunit vaccine against SARS-CoV-2 using immunoinformatics approach

In Silico Pharmacology - Tập 9 - Trang 1-21 - 2021
Elijah Kolawole Oladipo1,2, Ayodeji Folorunsho Ajayi3, Olugbenga Samson Onile4, Olumuyiwa Elijah Ariyo5, Esther Moradeyo Jimah6,2, Louis Odinakaose Ezediuno7,2, Oluwadunsin Iyanuoluwa Adebayo3,2, Emmanuel Tayo Adebayo3,2, Aduragbemi Noah Odeyemi3,2, Marvellous Oluwaseun Oyeleke3, Moyosoluwa Precious Oyewole8, Ayomide Samuel Oguntomi2, Olawumi Elizabeth Akindiya9, Victoria Oyetayo Aremu3,2, Dorcas Olubunmi Aboderin10, Julius Kola Oloke11
1Department of Microbiology, Laboratory of Molecular Biology, Immunology and Bioinformatics, Adeleke University, Ede, Nigeria
2Genomics Unit, Helix Biogen Consult, Ogbomoso, Nigeria
3Reproduction and Bioinformatics Unit, Department of Medical Physiology, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
4Biotechnology Programme, Department of Biological Sciences, Elizade University, Ilara-Mokin, Nigeria
5Department of Medicine, Infectious Diseases and Tropical Medicine Unit, Federal Teaching Hospital, Ido-Ekiti, Nigeria
6Department of Medical Microbiology and Parasitology, University of Ilorin, Ilorin, Nigeria
7Department of Microbiology and Parasitology, University of Ilorin, Ilorin, Nigeria
8Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
9Microbiology Programme, Department of Biological Science, Olusegun Agagu University of Science and Technology, Okitipupa, Nigeria
10Department of Medical Microbiology and Parasitology, University of Lagos, Lagos, Nigeria
11Department of Natural Sciences, Precious Cornerstone University, Ibadan, Nigeria

Tóm tắt

The widespread of coronavirus (COVID-19) is a new global health crisis that poses a threat to the world. The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) originated in bats and was discovered first in Wuhan, Hubei province, China in December 2019. Immunoinformatics and bioinformatics tools were employed for the construction of a multi-epitope subunit vaccine to prevent the diseases. The antigenicity, toxicity and allergenicity of all epitopes used in the construction of the vaccine were predicted and then conjugated with adjuvants and linkers. Vaccine Toll-Like Receptors (2, 3, 4, 8 and 9) complex was also evaluated. The vaccine construct was antigenic, non-toxic and non-allergic, which indicates the vaccines ability to induce antibodies in the host, making it an effective vaccine candidate.

Tài liệu tham khảo

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