Mô tả và phân tích gen của Microvirga antarctica sp. nov., một vi khuẩn tâm sắc hồng mới có khả năng chịu lạnh, được phân lập từ đất ở Nam Cực

Antonie van Leeuwenhoek - Tập 114 - Trang 2219-2228 - 2021
Lin Zhu1, Weiwei Ping1, Siyue Zhang1, Ya Chen1, Ying Zhang1, Jianli Zhang1
1Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, Beijing, People’s Republic of China

Tóm tắt

Một chủng vi khuẩn có sắc tố hồng mới, được ký hiệu là chủng 3D7T, đã được phân lập trong quá trình nghiên cứu các loài có khả năng chịu lạnh tiềm năng từ đất ở Nam Cực. Các tế bào của chủng phân lập được quan sát có dạng hình que (0.7–0.9 × 1.0–2.2 µm), nhuộm Gram âm và không di động. Nó có khả năng phát triển ở nhiệt độ 4–32 °C, pH 7.0–10.0 và trong sự hiện diện của 0–3% (w/v) NaCl. Phân tích phát sinh nghĩa dựa trên chuỗi gen 16S rRNA cho thấy chủng 3D7T thuộc về chi Microvirga và có liên quan chặt chẽ đến ‘Microvirga brassicacearum’ CDVBN77T (98.3%), Microvirga subterranea DSM 14364 T (96.8%), Microvirga guangxiensis 25BT (96.5%) và Microvirga aerophila DSM 21344 T (96.5%). Quinone chủ yếu là ubiquinone 10 (Q-10), và các axit béo chính là đặc trưng tập hợp 8 (C18:1ω7c và/hoặc C18:1ω6c) và C19:0 cyclo ω8c. Các lipid phân cực chủ yếu là phosphatidylcholine và phosphatidylethanolamine. Hàm lượng G + C của DNA toàn phần của chủng 3D7T là 63.5 mol%. Chuỗi gen của nó cho thấy các gen mã hóa phosphatase và lipase. Cơ chế di truyền liên quan đến enzyme hoạt động carbohydrate và các chất chuyển hóa thứ cấp cũng được quan sát. Giá trị đồng nhất nucleotide trung bình và giá trị lai DNA số cho thấy dựa trên chuỗi gen toàn bộ của chủng 3D7T và các loài liên quan chặt chẽ của nó nằm dưới ngưỡng xác định loài. Các phân tích kiểu hình, hóa phân loại, phát sinh nghĩa và gen cho thấy rằng chủng 3D7T đại diện cho một loài mới của chi Microvirga, cho loài này tên gọi Microvirga antarctica sp. nov. được đề xuất. Chủng loại là 3D7T (= CGMCC 1.13821T = KCTC 72465T).

Từ khóa

#Microvirga antarctica #vi khuẩn chịu lạnh #phân lập từ đất Nam Cực #phân tích gen #phylogenetics

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