Dengue 3 virus distribution in the mosquito Aedes aegyptl: an immunocytochemical study

Medical and Veterinary Entomology - Tập 10 Số 1 - Trang 87-92 - 1996
Khin Saw Aye Myint1, Kenneth B. Platt2, Kriangkrai Lerdthusnee3, B L. INNI4, D W. VAU GHN5
1Departments of Entomology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
2Department of Microbiology, Iowa State University, Ames, Iowa, U.S.A
3National Centre for Genetic Engineering and Biotechnology, Bangkok, Thailand
4Department of Virology, Walter Reed Army Institute of Research, Washington, D.C., U.S.A
5Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand

Tóm tắt

Abstract. The dissemination of dengue (DEN) 3 virus in parenterally infected female Aedes aegypti mosquitoes was studied imrnunocytochemically. Antigen was first detected in fat body cells near the thoracic site of virus inoculation. The intussuscepted foregut, salivary glands and nervous tissue were the first major tissues infected. Nervous tissue appeared to be the primary site of amplification. Muscles, tracheae, Malphigian tubules and the posterior midgut did not become infected. The only part of the reproductive system to be infected was the calyx (71% of specimens 16–22 days post‐infection) consistent with low rates of vertical transmission. After 7 days post‐inoculation the salivary glands of 100% of the specimens examined were infected. Virus dissemination was slow and the most common sequence of infection following intrathoracic inoculation was as follows: thoracic fat body, intussuscepted foregut, salivary glands, cardial epithelium, thoracic ganglion, brain, compound eye, anterior midgut, intermediate midgut/anterior abdominal ganglia, and calyx/hindgut/posterior abdominal ganglia. Fat body and intussuscepted foregut tissues lost infections after 16 days post‐inoculation.

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Medical and Veterinary Entomology

Tập 10 Số 1

87-92

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