Jaka Razinger1, Luka Drinovec2, Maja Berden-Zrimec3
1Agricultural Institute of Slovenia, 1000, Ljubljana, Slovenia
2Aerosol d.o.o, 1000, Ljubljana, Slovenia
3Institute of Physical Biology, 1000, Ljubljana, Slovenia
Tóm tắt
AbstractEarly chemical-induced stress in Solanum tuberosum leaves was visualized using delayed fluorescence (DF) imaging. The ability to detect spatially heterogeneous responses of plant leaves exposed to several toxicants using delayed fluorescence was compared to prompt fluorescence (PF) imaging and the standard maximum fluorescence yield of PSII measurements (Fv/Fm). The toxicants used in the study were two photosynthesis inhibitors (herbicides), 100 µM methyl viologen (MV) and 140 µM diuron (DCMU), and two heavy metals, 100 µM cadmium and 100 µM copper. The exposure times were 5 and 72 h. Significant photosynthesis-inhibitor effects were already visualized after 5 h. In addition, a significant reduction in the DF/PF index was measured in DCMU- and MV-treated leaves after 5 h. In contrast, only DCMU-treated leaves exhibited a significant decrease in Fv/Fm after 5 h. All treatments resulted in a significant decrease in the DF/PF parameter after 72 h of exposure, when only MV and Cd treatment resulted in visible symptoms. Our study highlights the power of delayed fluorescence imaging. Abundant quantifiable spatial information was obtained with the instrumental setup. Delayed fluorescence imaging has been confirmed as a very responsive and useful technique for detecting stress induced by photosynthesis inhibitors or heavy metals.
