Decontamination of Hospital Surfaces With Multijet Cold Plasma: A Method to Enhance Infection Prevention and Control?

Infection Control and Hospital Epidemiology - Tập 38 Số 10 - Trang 1182-1187 - 2017
Orla Cahill1,2, Tânia Claro3, A.A. Cafolla4, Niall Stevens3, Stephen Daniels1, Hilary Humphreys3,5
1Department of Food Science and Environmental Health, Dublin Institute of Technology, Dublin, Ireland
2School of Electronic Engineering and National Centre for Plasma Science Technology, Dublin City University, Dublin, Ireland
3Department of Clinical Microbiology, Royal College of Surgeons in Ireland, Dublin, Ireland
4School of Physical Sciences, Dublin City University, Dublin, Ireland
5Department of Microbiology, Beaumont Hospital, Dublin, Ireland

Tóm tắt

OBJECTIVETo evaluate the efficacy of a multijet cold-plasma system and its efficacy in decontaminating 2 surfaces commonly found in hospitalsDESIGNAn in vitro study of common causes of healthcare-acquired infectionMETHODSLog10 9 cultures of methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, extended spectrum β-lactamase–producing Escherichia coli, and Acinetobacter baumannii were applied to 5-cm2 sections of stainless steel and mattress. Human serum albumin (HSA) was used as a proxy marker for organic material, and atomic force microscopy (AFM) was used to study the impact on bacterial cell structure. The inoculated surfaces were exposed to a cold-air-plasma–generating multijet prototype for 15, 20, 30, and 45 seconds.RESULTSAfter 45 seconds, at least 3 to 4 log reductions were achieved for all bacteria on the mattress, while 3 to 6 log reductions were observed on stainless steel. The presence of HSA had no appreciable effect on bacterial eradication. The surfaces with bacteria exposed to AFM showed significant morphological changes indicative of “etching” due to the action of highly charged ions produced by the plasma.CONCLUSIONThis multijet cold-plasma prototype has the potential to augment current environmental decontamination approaches but needs further evaluation in a clinical setting to confirm its effectiveness.Infect Control Hosp Epidemiol 2017;38:1182–1187

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Thông tin
Thông tin xuất bản

Infection Control and Hospital Epidemiology

Tập 38 Số 10

1182-1187

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