Dark chocolate: An overview of its biological activity, processing, and fortification approaches

Abt, 2020, Perspective on cadmium and lead in cocoa and chocolate, J. Agric. Food Chem., 68, 13008, 10.1021/acs.jafc.9b08295 Adam, 2002, The bioavailability of ferulic acid is governed primarily by the food matrix rather than its metabolism in intestine and liver in rats, J. Nutr., 132, 1962, 10.1093/jn/132.7.1962 Ader, 2001, Interaction of quercetin glucosides with the intestinal sodium/glucose co-transporter (SGLT-1), Cancer Lett., 162, 175, 10.1016/S0304-3835(00)00645-5 Adeyeye, 2010, Effect of farm and industrial processing on the amino acid profile of cocoa beans, Food Chem., 118, 357, 10.1016/j.foodchem.2009.04.127 Afoakwa, 2007, Factors influencing rheological and textural qualities in chocolate - a review, Trends Food Sci. Technol., 18, 290, 10.1016/j.tifs.2007.02.002 Afoakwa, 2008, Characterization of melting properties in dark chocolates from varying particle size distribution and composition using differential scanning calorimetry, Food Res. Int., 41, 751, 10.1016/j.foodres.2008.05.009 Afoakwa, 2008, Flavor formation and character in cocoa and chocolate: a critical review, Crit. Rev. Food Sci. Nutr., 48, 840, 10.1080/10408390701719272 Afoakwa, 2009, Fat bloom development and structure-appearance relationships during storage of under-tempered dark chocolates, J. Food Eng., 91, 571, 10.1016/j.jfoodeng.2008.10.011 Afoakwa, 2013, Chemical composition and physical quality characteristics of Ghanaian cocoa beans as affected by pulp pre-conditioning and fermentation, J. Food Sci. Technol., 50, 1097, 10.1007/s13197-011-0446-5 Aidoo, 2017, Functionality of inulin and polydextrose as sucrose replacers in sugar-free dark chocolate manufacture – effect of fat content and bulk mixture concentration on rheological, mechanical and melting properties, Int. J. Food Sci. Technol., 52, 282, 10.1111/ijfs.13281 Akkarachiyasit, 2010, Inhibitory activities of cyanidin and its glycosides and synergistic effect with acarbose against intestinal α-glucosidase and pancreatic α-amylase, Int. J. Mol. Sci., 11, 3387, 10.3390/ijms11093387 Albak, 2015, Effect of cinnamon powder addition during conching on the flavor of dark chocolate mass, J. Food Sci. Technol., 52, 1960, 10.1007/s13197-013-1217-2 Albak, 2016, Variation of total aroma and polyphenol content of dark chocolate during three phase of conching, J. Food Sci. Technol., 53, 848, 10.1007/s13197-015-2036-4 Almatroodi, 2021, Potential therapeutic targets of quercetin, a plant flavonol, and its role in the therapy of various types of cancer through the modulation of various cell signaling pathways, Molecules, 26, 1315, 10.3390/molecules26051315 Andújar, 2012, Cocoa polyphenols and their potential benefits for human health, Oxid. Med. Cell. Longev., 2012, 10.1155/2012/906252 Appeldoorn, 2009, Procyanidin dimers A1, A2, and B2 are absorbed without conjugation or methylation from the small intestine of rats, J. Nutr., 139, 1469, 10.3945/jn.109.106765 Aprotosoaie, 2016, Flavor chemistry of cocoa and cocoa products-an overview, Compr. Rev. Food Sci. Food Saf., 15, 73, 10.1111/1541-4337.12180 Araujo, 2016, Cocoa and human health: from head to foot—a review, Crit. Rev. Food Sci. Nutr., 56, 1, 10.1080/10408398.2012.657921 Aremu, 1995, Nutrient and antinutrient profiles of raw and fermented cocoa beans, Plant Foods Hum. Nutr., 48, 217, 10.1007/BF01088443 Ashihara, 2011, Distribution, biosynthesis and catabolism of methylxanthines in plants, Handb. Exp. Pharmacol., 200, 11, 10.1007/978-3-642-13443-2_2 Asti, 2020, vol. 147 Azuma, 2000, Absorption of chlorogenic acid and caffeic acid in rats after oral administration, J. Agric. Food Chem., 48, 5496, 10.1021/jf000483q Baba, 2000, Bioavailability of (-)-epicatechin upon intake of chocolate and cocoa in human volunteers, Free Radic. Res., 33, 635, 10.1080/10715760000301151 Ballard, 2018, Health benefits of flavonoids Barišić, 2019, The chemistry behind chocolate production, Molecules, 24, 10.3390/molecules24173163 Baron, 1999, Hemodynamic and electrophysiologic effects of acute chocolate ingestion in young adults, Am. J. Cardiol., 84, 370, 10.1016/S0002-9149(99)00301-X Barrio, 2020, Improvement of the flavanol profile and the antioxidant capacity of chocolate using a phenolic rich cocoa powder, Foods, 9, 1 Bartkiene, 2021, The evaluation of dark chocolate-elicited emotions and their relation with physico chemical attributes of chocolate, Foods, 10, 1, 10.3390/foods10030642 Beach, 1986, Inhibition of elimination of caffeine by disulfiram in normal subjects and recovering alcoholics, Clin. Pharmacol. Ther., 39, 265, 10.1038/clpt.1986.37 Blanco-Montenegro, 2007, Imaging and modelling the subsurface structure of volcanic calderas with high-resolution aeromagnetic data at Vulcano (Aeolian Islands, Italy), Bull. Volcanol., 69, 643, 10.1007/s00445-006-0100-7 Blinks, 1972, Influence of caffeine and other methylxanthines on mechanical properties of isolated mammalian heart muscle, Circulation, 367, 10.1161/01.RES.30.4.367 Bölek, 2022, Effects of Santolina chamaecyparissus essential oil on rheological, thermal and antioxidative properties of dark chocolate, International Journal of Gastronomy and Food Science, 27 Botelho, 2014, Oxidative stability of functional phytosterol-enriched dark chocolate, LWT - Food Sci. Technol. (Lebensmittel-Wissenschaft -Technol.), 55, 444, 10.1016/j.lwt.2013.09.002 Brglez Mojzer, 2016, Polyphenols: extraction methods, antioxidative action, bioavailability and anticarcinogenic effects, Molecules, 21, 10.3390/molecules21070901 Burgos, 2014, The cyclic antibacterial peptide enterocin AS-48: isolation, mode of action, and possible food applications, Int. J. Mol. Sci., 15, 22706, 10.3390/ijms151222706 Caprioli, 2016, Nutritional composition, bioactive compounds and volatile profile of cocoa beans from different regions of Cameroon, Int. J. Food Sci. Nutr., 67, 422, 10.3109/09637486.2016.1170769 Charoenngam, 2020, Immunologic effects of vitamin d on human health and disease, Nutrients, 12, 1, 10.3390/nu12072097 Cheng, 2015, The impact of variety, environment and agricultural practices on catechins and caffeine in plucked tea leaves, Process. Impact on Active Components in Food, 597, 10.1016/B978-0-12-404699-3.00072-X Chen, 2017, Astaxanthin reduces MMP expressions, suppresses cancer cell migrations, and triggers apoptotic caspases of in vitro and in vivo models in melanoma, J. Funct.Foods, 31, 20, 10.1016/j.jff.2017.01.005 Chiang, 2006, Antioxidant effects of black rice extract through the induction of superoxide dismutase and catalase activities, Lipids, 41, 797, 10.1007/s11745-006-5033-6 Clewes, 2013, Food fortification, Nutrition in Infancy, 1, 359, 10.1007/978-1-62703-224-7_25 Cooper, 2007, Understanding in multinational organizations, J. Organ. Behav., 28, 303, 10.1002/job.440 Cooper, 2008, Predictive relationship between polyphenol and nonfat cocoa solids content of chocolate, J. Agric. Food Chem., 56, 260, 10.1021/jf072153c Curtis, 2012, Chronic ingestion of flavan-3-ols and isoflavones improves insulin sensitivity and lipoprotein status and attenuates estimated 10-year CVD risk in medicated postmenopausal women with type 2 diabetes: a 1-year, double-blind, randomized,controlled trial, Diabetes Care, 35, 226, 10.2337/dc11-1443 Cvitanovic, 2012, Innovative formulations of chocolates enriched with plant polyphenols from Rubus idaeus L. leaves and characterization of their physical, bioactive and sensory properties, Food Res. Int., 48, 820, 10.1016/j.foodres.2012.06.023 Czank, 2013, Human metabolism and elimination of the anthocyanin, cyanidin-3-glucoside: a 13C-tracer study, Am. J. Clin. Nutr., 97, 995, 10.3945/ajcn.112.049247 Davison, 2012, The effect of acute pre-exercise dark chocolate consumption on plasma antioxidant status, oxidative stress and immunoendocrine responses to prolonged exercise, Eur. J. Nutr., 51, 69, 10.1007/s00394-011-0193-4 Day, 2003, Absorption of quercetin-3-glucoside and quercetin-4′-glucoside in the rat small intestine: the role of lactase phlorizin hydrolase and the sodium-dependent glucose transporter, Biochem. Pharmacol., 65, 1199, 10.1016/S0006-2952(03)00039-X Decroix, 2017, Acute cocoa Flavanols intake has minimal effects on exercise-induced oxidative stress and nitric oxide production in healthy cyclists: a randomized controlled trial, Sports Nutr. Rev. J., 14 Delgado, 2018, Role of prebiotics in regulation of microbiota and prevention of obesity, Food Res. Int., 113, 183, 10.1016/j.foodres.2018.07.013 Desideri, 2012, Benefits in cognitive function, blood pressure, and insulin resistance through cocoa flavanol consumption in elderly subjects with mild cognitive impairment: the cocoa, cognition, and aging (CoCoA) study, Hypertension, 60, 794, 10.1161/HYPERTENSIONAHA.112.193060 Didar, 2021, Enrichment of dark chocolate with vitamin D3 (free or liposome) and assessment quality parameters, J. Food Sci. Technol., 58, 3065, 10.1007/s13197-020-04810-x Dillinger, 2000, Food of the Gods: cure for humanity? A cultural history of the medicinal and ritual use of chocolate, J. Nutr., 130, 2057, 10.1093/jn/130.8.2057S Djikeng, 2018, Effect of traditional and oven roasting on the physicochemical properties of fermented cocoa beans, Heliyon, 4, 10.1016/j.heliyon.2018.e00533 Dugo, 2017, Effect of cocoa polyphenolic extract on macrophage polarization from proinflammatory M1 to anti-inflammatory M2 state, Oxid. Med. Cell. Longev., 2017, 10.1155/2017/6293740 Dwijatmokoa, 2016, Effect of cinnamon essential oils addition in the sensory attributes of dark chocolate, Nusantara Bioscience, 8, 301, 10.13057/nusbiosci/n080227 Ekantari, 2019, Stability of chocolate bars fortified with nanocapsules carotenoid of Spirulina platensis, IOP Conf. Ser. Earth Environ. Sci., 370, 10.1088/1755-1315/370/1/012079 Engler, 2006, The emerging role of flavonoid-rich cocoa and chocolate in cardiovascular health and disease, Nutr. Rev., 64, 109, 10.1111/j.1753-4887.2006.tb00194.x Engler, 2004, Flavonoid-rich dark chocolate improves endothelial function and increases plasma epicatechin concentrations in healthy adults, J. Am. Coll. Nutr., 23, 197, 10.1080/07315724.2004.10719361 Erdem, 2014, Development of a novel synbiotic dark chocolate enriched with Bacillus indicus HU36, maltodextrin and lemon fiber: optimization by response surface methodology, LWT - Food Sci. Technol. (Lebensmittel-Wissenschaft -Technol.), 56, 187, 10.1016/j.lwt.2013.10.020 Erdman, 2008, Effects of cocoa flavanols on risk factors for cardiovascular disease, Asia Pac. J. Clin. Nutr., 17, 284 Faccinetto-Beltrán, 2021, Chocolate as carrier to deliver bioactive ingredients: current advances and future perspectives, Foods, 10, 1, 10.3390/foods10092065 Fakhri, 2018, Astaxanthin: a mechanistic review on its biological activities and health benefits, Pharmacol. Res., 136, 1, 10.1016/j.phrs.2018.08.012 Fanton, 2021, The sweet side of dark chocolate for chronic kidney disease patients, Clin. Nutr., 40, 15, 10.1016/j.clnu.2020.06.039 Farhat, 2014, Dark chocolate: an obesity paradox or a culprit for weight gain?, Phytother Res., 28, 791, 10.1002/ptr.5062 Fernández, 2021, Physicochemical properties and sensory acceptability of sugar-free dark chocolate formulations added with probiotics, Rev. Mex. Ing. Quim., 20, 697, 10.24275/rmiq/Alim2131 Fisher, 2005, Flavanols for cardiovascular health: the science behind the sweetness, J. Hypertens., 23, 1453, 10.1097/01.hjh.0000174605.34027.9d Flammer, 2007, Dark chocolate improves coronary vasomotion and reduces platelet reactivity, Circulation, 116, 2376, 10.1161/CIRCULATIONAHA.107.713867 Gentili, 2013, Comprehensive profiling of carotenoids and fat-soluble vitamins in milk from different animal species by LC-DAD-MS/MS hyphenation, J. Agric. Food Chem., 61, 1628, 10.1021/jf302811a Gianfredi, 2018, Can chocolate consumption reduce cardio-cerebrovascular risk? A systematic review and meta-analysis, Nutrition, 46, 103, 10.1016/j.nut.2017.09.006 Godočiková, 2017, The influence of fortification of dark chocolate with Sea buckthorn and mulberry on the content of biologically active substances, Advanced Research in Life Sciences, 1, 26, 10.1515/arls-2017-0004 Goldin, 1998, Health benefits of probiotics, Br. J. Nutr., 80 Grassi, 2005, Cocoa reduces blood pressure and insulin resistance and improves endothelium-dependent vasodilation in hypertensives, Hypertension, 46, 398, 10.1161/01.HYP.0000174990.46027.70 Grassi, 2008, Blood pressure is reduced and insulin sensitivity increased in glucose-intolerant, hypertensive subjects after 15 days of consuming high-polyphenol dark chocolate, J. Nutr., 138, 1671, 10.1093/jn/138.9.1671 Grassi, 2005, Short-term administration of dark chocolate is followed by a significant increase in insulin sensitivity and a decrease in blood pressure in healthy persons, Am. J. Clin. Nutr., 81, 611, 10.1093/ajcn/81.3.611 Gruenwald, 2010, Cinnamon and health, Crit. Rev. Food Sci. Nutr., 50, 822, 10.1080/10408390902773052 Habanova, 2016, Intake of bilberries (Vaccinium myrtillus L.) reduced risk factors for cardiovascular disease by inducing favorable changes in lipoprotein profiles, Nutr. Res., 36, 1415, 10.1016/j.nutres.2016.11.010 Hannum, 2000, Emerging health benefits from cocoa and chocolate, J. Med. Food, 3, 73, 10.1089/109662000416276 Hapsari, 2020, The determinant factors of Indonesian competitiveness of cocoa exports to Germany, Jurnal Ekonomi Pembanguna, 18, 75, 10.29259/jep.v18i1.9978 Haritha, 2014, Health benefits of dark chocolate, Journal of Advanced Drug Delivery, 1, 184 Heinrich, 2006, Long-term ingestion of high flavanol cocoa provides photoprotection against UV-induced erythema and improves skin condition in women, J. Nutr., 136, 1565, 10.1093/jn/136.6.1565 Henderson, 2019, Chasing chocolate, 15 Hollenberg, 2006, Vascular action of cocoa flavanols in humans: the roots of the story, J. Cardiovasc. Pharmacol., 47, 99, 10.1097/00005344-200606001-00002 Hooper, 2012, Effects of chocolate, cocoa, and flavan-3-ols on cardiovascular health: a systematic review and meta-analysis of randomized trials, Am. J. Clin. Nutr., 95, 740, 10.3945/ajcn.111.023457 Hurst, 2008, Survey of the trans-resveratrol and trans-piceid content of cocoa-containing and chocolate products, J. Agric. Food Chem., 56, 8374, 10.1021/jf801297w Ibrahim, 2020, Quantification of physicochemical and microstructure properties of dark chocolate incorporated with palm sugar and dates as alternative sweetener, Mater. Today: Proc., 31, 366 Ioannone, 2015, Flavanols, proanthocyanidins and antioxidant activity changes during cocoa (Theobroma cacao L.) roasting as affected by temperature and time of processing, Food Chem., 174, 256, 10.1016/j.foodchem.2014.11.019 Jalil, 2008, Polyphenols in cocoa and cocoa products: is there a link between antioxidant properties and health?, Molecules, 13, 2190, 10.3390/molecules13092190 Jara, 2018, Impact of dietary Arthrospira (Spirulina) biomass consumption on human health: main health targets and systematic review, J. Appl. Phycol., 30, 2403, 10.1007/s10811-018-1468-4 Jawad, 2013, Perfil de eficacia y seguridad de Echinacea purpurea en la prevención de episodios de resfriado común: Estudio clínico aleatorizado, doble ciego y controlado con placebo, Revista de Fitoterapia, 13, 125 Karakaya, 2004, Bioavailability of phenolic compounds, Crit. Rev. Food Sci. Nutr., 44, 453, 10.1080/10408690490886683 Keith, 2019, Cinnamon: update of potential health benefits, Nutr. Today, 54, 42, 10.1097/NT.0000000000000319 Kemsawasd, 2016, Survival of immobilized probiotics in chocolate during storage and with an in vitro gastrointestinal model, Food Biosci., 16, 37, 10.1016/j.fbio.2016.09.001 Kerimi, 2015, The cardiovascular benefits of dark chocolate, Vasc. Pharmacol., 71, 11, 10.1016/j.vph.2015.05.011 Kobus-Cisowska, 2019, Enriching novel dark chocolate with Bacillus coagulans as a way to provide beneficial nutrients, Food Funct., 10, 997, 10.1039/C8FO02099J Konar, 2013, Influence of conching temperature and some bulk sweeteners on physical and rheological properties of prebiotic milk chocolate containing inulin, Eur. Food Res. Technol., 236, 135, 10.1007/s00217-012-1873-x Konar, 2018, Enrichment of milk chocolate by using EPA and DHA originated from various origins: effects on product quality, Sugar Tech, 20, 745, 10.1007/s12355-018-0611-5 Konishi, 2006, Phenolic acids are absorbed from the rat stomach with different absorption rates, J. Agric. Food Chem., 54, 7539, 10.1021/jf061554+ Kord-Varkaneh, 2019, Does cocoa/dark chocolate supplementation have favorable effect on body weight, body mass index and waist circumference? A systematic review, meta-analysis and dose-response of randomized clinical trials, Crit. Rev. Food Sci. Nutr., 59, 2349, 10.1080/10408398.2018.1451820 Kropat, 2013, Modulation of Nrf2-dependent gene transcription by bilberry anthocyanins in vivo, Mol. Nutr. Food Res., 57, 545, 10.1002/mnfr.201200504 Kruger, 2014, Proanthocyanidins, anthocyanins and cardiovascular diseases, Food Res. Int., 59, 41, 10.1016/j.foodres.2014.01.046 Krysiak, 2006, Influence of roasting conditions on coloration of roasted cocoa beans, J. Food Eng., 77, 449, 10.1016/j.jfoodeng.2005.07.013 Krysiak, 2011, Effects of convective and microwave roasting on the physicochemical properties of cocoa beans and cocoa butter extracted from this material, Grasas Aceites, 62, 467, 10.3989/gya.114910 Kuntz, 2014, Anthocyanins from fruit juices improve the antioxidant status of healthy young female volunteers without affecting anti-inflammatory parameters: results from the randomised, double-blind, placebo-controlled, cross-over ANTHONIA (ANTHOcyanins in Nutrition, Br. J. Nutr., 112, 925, 10.1017/S0007114514001482 Lamport, 2020, Beneficial effects of dark chocolate for episodic memory in healthy young adults: a parallel-groups acute intervention with a white chocolate control, Nutrients, 12, 10.3390/nu12020483 Lechtenberg, 2012, Fast determination of N-phenylpropenoyl-l-amino acids (NPA) in cocoa samples from different origins by ultra-performance liquid chromatography and capillary electrophoresis, Food Chem., 135, 1676, 10.1016/j.foodchem.2012.06.006 Lecumberri, 2007, A diet rich in dietary fiber from cocoa improves lipid profile and reduces malondialdehyde in hypercholesterolemic rats, Nutrition, 23, 332, 10.1016/j.nut.2007.01.013 Ligęza, 2019, A comparative study of thermal and textural properties of milk, white and dark chocolates, Thermochim. Acta, 671, 60, 10.1016/j.tca.2018.11.005 Lillah, 2017, Improving heat stability along with quality of compound dark chocolate by adding optimized cocoa butter substitute (hydrogenated palm kernel stearin) emulsion, LWT - Food Sci. Technol. (Lebensmittel-Wissenschaft -Technol.), 80, 531, 10.1016/j.lwt.2017.02.042 Lippi, 2009, Dark chocolate: consumption for pleasure or therapy?, J. Thromb. Thrombolysis, 28, 482, 10.1007/s11239-008-0273-3 Lorenzo, 2022, Structure and nutrition of dark chocolate with pequi mesocarp (Caryocar villosum (Alb.) Pers.), Food Sci. Technol., 42, 10.1590/fst.88021 Manach, 2004, Polyphenols: food sources and bioavailability, Am. J. Clin. Nutr., 79, 727, 10.1093/ajcn/79.5.727 Mao, 2000, The effect of cocoa procyanidins on the transcription and secretion of interleukin 1β in peripheral blood mononuclear cells, Life Sci., 66, 1377, 10.1016/S0024-3205(00)00449-5 Marazeeq, 2018, Evaluation of proximate composition and sensory attributes of dark chocolate fortified with wheat germ, Adv. J. Food Sci. Technol., 14, 103, 10.19026/ajfst.14.5843 Marie, 2021, Benefits of polyphenols and methylxanthines from cocoa, Food, 10, 1 Martini, 2018, Comprehensive evaluation of phenolic profile in dark chocolate and dark chocolate enriched with Sakura green tea leaves or turmeric powder, Food Res. Int., 112, 1, 10.1016/j.foodres.2018.06.020 Matsui, 2003, From red microalgae have antiinflammatory properties in vitro and in vivo, Appl. Biochem. Biotechnol., 104, 13, 10.1385/ABAB:104:1:13 Mattia, 2013, Effect of fermentation and drying on procyanidins, antiradical activity and reducing properties of cocoa beans, Food Bioprocess Technol., 6, 3420, 10.1007/s11947-012-1028-x Mattia, 2017, From cocoa to chocolate: the impact of processing on in vitro antioxidant activity and the effects of chocolate on antioxidant markers in vivo, Front. Immunol., 8, 1 Mattia, 2014, Effect of different conching processes on procyanidin content and antioxidant properties of chocolate, Food Res. Int., 63, 367, 10.1016/j.foodres.2014.04.009 McShea, 2008, Clinical benefit and preservation of flavonols in dark chocolate manufacturing, Nutr. Rev., 66, 630, 10.1111/j.1753-4887.2008.00114.x Menesi, 2009, Dietary reference intakes for DHA and EPA, Prostaglandins Leukot. Essent. Fatty Acids, 81, 99 Mennen, 2006, Urinary flavonoids and phenolic acids as biomarkers of intake for polyphenol-rich foods, Br. J. Nutr., 96, 191, 10.1079/BJN20061808 Mink, 2007, Flavonoid intake and cardiovascular disease mortality: a prospective study in postmenopausal women, Am. J. Clin. Nutr., 85, 895, 10.1093/ajcn/85.3.895 Mirković, 2018, The sensory quality and volatile profile of dark chocolate enriched with encapsulated probiotic lactobacillus plantarum bacteria, Sensors, 18, 10.3390/s18082570 Mišurcová, 2012, Health benefits of algal polysaccharides in human nutrition, vol. 66 Montagnana, 2018, Dark chocolate modulates platelet function with a mechanism mediated by flavan-3-ol metabolites, Medicine (United States), 97, 1 Moreno, 2012, Dark chocolate acceptability: influence of cocoa origin and processing conditions, J. Sci. Food Agric., 92, 404, 10.1002/jsfa.4592 Munoz, 2013, Prooxidant and antioxidant activities of rosmarinic acid, J. Food Biochem., 37, 396, 10.1111/j.1745-4514.2011.00639.x Murga, 2011, The impact of chocolate on cardiovascular health, Maturitas, 69, 312, 10.1016/j.maturitas.2011.05.011 Nagaraj, 2012, Antiproliferative potential of astaxanthin-rich alga Haematococcus pluvialis Flotow on human hepatic cancer (HepG2) cell line, Biomedicine and Preventive Nutrition, 2, 149, 10.1016/j.bionut.2012.03.009 Nastaj, 2022, Development and physicochemical properties of reformulated, high-protein, untempered sugar-free dark chocolates with addition of whey protein isolate and erythritol. IInt, Dairy J., 134, 10.1016/j.idairyj.2022.105450 Nebesny, 2007, Dark chocolates supplemented with Lactobacillus strains, Eur. Food Res. Technol., 225, 33, 10.1007/s00217-006-0379-9 Németh, 2003, Deglycosylation by small intestinal epithelial cell β-glucosidases is a critical step in the absorption and metabolism of dietary flavonoid glycosides in humans, Eur. J. Nutr., 42, 29, 10.1007/s00394-003-0397-3 Neufingerl, 2013, Effect of cocoa and theobromine consumption on serum HDL-cholesterol concentrations: a randomized controlled trial, Am. J. Clin. Nutr., 97, 1201, 10.3945/ajcn.112.047373 Neukam, 2007, Consumption of flavanol-rich cocoa acutely increases microcirculation in human skin, Eur. J. Nutr., 46, 53, 10.1007/s00394-006-0627-6 Ngamdee, 2020, Development and study on physical and sensory properties of dark chocolates fortified with anthocyanin from broken, RMUTP Research Journal, 14, 45 Nicole, 2005, Role of flavonoids in oxidative stress, Curr. Top. Med. Chem., 1, 569, 10.2174/1568026013394750 Nogueira, 2012, Consumption of high-polyphenol dark chocolate improves endothelial function in individuals with stage 1 hypertension and excess body weight, Int. J. Hypertens., 2012, 10.1155/2012/147321 Norhayati, 2013, Effect of storage conditions on quality of prebiotic dark chocolate, Malaysian Journal of Nutrition, 19, 111 Nunes, 2017, Therapeutic and nutraceutical potential of rosmarinic acid—cytoprotective properties and pharmacokinetic profile, Crit. Rev. Food Sci. Nutr., 57, 1799 Oracz, 2016, Antioxidant properties of cocoa beans (theobroma cacao L.): influence of cultivar and roasting conditions, Int. J. Food Prop., 19, 1242, 10.1080/10942912.2015.1071840 Ostertag, 2013, Flavan-3-ol-enriched dark chocolate and white chocolate improve acute measures of platelet function in a gender-specific way-a randomized-controlled human intervention trial, Mol. Nutr. Food Res., 57, 191, 10.1002/mnfr.201200283 Ostertag, 2017, Acute consumption of flavan-3-ol-enriched dark chocolate affects human endogenous metabolism, J. Proteome Res., 16, 2516, 10.1021/acs.jproteome.7b00089 Ou, 2012, Transport of cranberry A-type procyanidin dimers, trimers, and tetramers across monolayers of human intestinal epithelial caco-2 cells, J. Agric. Food Chem., 60, 1390, 10.1021/jf2040912 Owusu, 2012, Effect of fermentation method, roasting and conching conditions on the aroma volatiles of dark chocolate, J. Food Process. Preserv., 36, 446, 10.1111/j.1745-4549.2011.00602.x Ozguven, 2016, Influence of processing conditions on procyanidin profiles and antioxidant capacity of chocolates: optimization of dark chocolate manufacturing by response surface methodology, LWT - Food Sci. Technol. (Lebensmittel-Wissenschaft -Technol.), 66, 252, 10.1016/j.lwt.2015.10.047 Ozguven, 2016, Fortification of dark chocolate with spray dried black mulberry (Morus nigra) waste extract encapsulated in chitosan-coated liposomes and bioaccessability studies, Food Chem., 201, 205, 10.1016/j.foodchem.2016.01.091 Parker, 2006, Mood state effects of chocolate, J. Affect. Disord., 92, 149, 10.1016/j.jad.2006.02.007 Permatasari, 2022, Kombucha drink enriched with sea grapes (Caulerpa racemosa) as potential functional beverage to contrast obesity: an in vivo and in vitro approach, Clinical Nutrition ESPEN, 49, 232, 10.1016/j.clnesp.2022.04.015 Permatasari, 2021, Kombucha tea from seagrapes (Caulerpa racemosa) potential as a functional anti-ageing food: in vitro and in vivo study, Heliyon, 7, 10.1016/j.heliyon.2021.e07944 Permatasari, 2022, Modulation of gut microbiota and markers of metabolic syndrome in mice on cholesterol and fat enriched diet by butterfly pea flower kombucha, Current Research in Food Science, 5, 1251, 10.1016/j.crfs.2022.08.005 Petyaev, 2018, Astaxanthin Co-crystallized with dark chocolate causes a dose-dependent inhibition of oxidation markers in middle-aged volunteers, American Journal of Food and Nutrition, 6, 153, 10.12691/ajfn-6-5-3 Pinilla, 2015, The relevance of theobromine for the beneficial effects of cocoa consumption, Front. Pharmacol., 6, 1 Planells, 1999, Ability of a cocoa product to correct chronic Mg deficiency in rats, Int. J. Vitam. Nutr. Res., 69, 52, 10.1024/0300-9831.69.1.52 Poliński, 2021, Physicochemical, antioxidant, microstructural properties and bioaccessibility of dark chocolate with plant extracts, Molecules, 26, 1, 10.3390/molecules26185523 Poveda, 2020, Cocoa bean shell — a by-product with nutritional, Nutrients, 12, 1 Praseptiangga, 2019, Sensory and physicochemical characteristics of dark chocolate bar with addition of cinnamon (Cinnamomum burmannii) bark oleoresin microcapsule, J. Food Sci. Technol., 56, 4323, 10.1007/s13197-019-03901-8 Quílez, 2003, Potential uses and benefits of phytosterols in diet: present situation and future directions, Clin. Nutr., 22, 343, 10.1016/S0261-5614(03)00060-8 Rahman, 2016 Ramadhanti, 2021, Projective mapping of preferences on milk and dark chocolate bar fortified nanocapsules Arthrospira carotenoid, IOP Conf. Ser. Earth Environ. Sci., 919, 10.1088/1755-1315/919/1/012031 Rao, 2013, Effective inhibition of skin cancer, tyrosinase, and antioxidative properties by astaxanthin and astaxanthin esters from the green alga haematococcus pluvialis, J. Agric. Food Chem., 61, 3842, 10.1021/jf304609j Rauf, 2018, Resveratrol as an anti-cancer agent: a review, Crit. Rev. Food Sci. Nutr., 58, 1428, 10.1080/10408398.2016.1263597 Reddy, 1999, The impact of food processing on the nutritional quality of vitamins and minerals, Adv. Exp. Med. Biol., 459, 99, 10.1007/978-1-4615-4853-9_7 Redgwell, 2003, Cocoa bean carbohydrates: roasting-induced changes and polymer interactions, Food Chem., 80, 511, 10.1016/S0308-8146(02)00320-5 Reid, 2020 Richelle, 1999, Plasma kinetics in man of epicatechin from black chocolate, Eur. J. Clin. Nutr., 53, 22, 10.1038/sj.ejcn.1600673 Roberfroid, 2000, Prebiotics and probiotics: are they functional foods?, Am. J. Clin. Nutr., 71, 1682, 10.1093/ajcn/71.6.1682S Roobab, 2020, Sources, formulations, advanced delivery and health benefits of probiotics, Curr. Opin. Food Sci., 32, 17, 10.1016/j.cofs.2020.01.003 Ruxton, 2004, The health benefits of omega-3 polyunsaturated fatty acids: a review of the evidence, J. Hum. Nutr. Diet., 17, 449, 10.1111/j.1365-277X.2004.00552.x Salvador, 2019, Can we conserve trans-resveratrol content and antioxidant activity during industrial production of chocolate?, J. Sci. Food Agric., 99, 83, 10.1002/jsfa.9146 Sánchez-Rabaneda, 2003, Liquid chromatographic/electrospray ionization tandem mass spectrometric study of the phenolic composition of cocoa (Theobroma cacao), J. Mass Spectrom., 38, 35, 10.1002/jms.395 Saraf, 2010, Phytoconstituents as photoprotective novel cosmetic formulations, Phcog. Rev., 4, 1, 10.4103/0973-7847.65319 Scapagnini, 2012, Antioxidants as antidepressants, CNS Drugs, 26, 477, 10.2165/11633190-000000000-00000 Schroeter, 2006, (-)-Epicatechin mediates beneficial effects of flavanol-rich cocoa on vascular function in humans, Proc. Natl. Acad. Sci. U. S. A., 103, 1024, 10.1073/pnas.0510168103 Schumacher, 2009, Development and evaluation of a laboratory scale conch for chocolate production, Int. J. Food Sci. Technol., 44, 616, 10.1111/j.1365-2621.2008.01877.x Schwan, 1998, Cocoa fermentations conducted with a defined microbial cocktail inoculum, Appl. Environ. Microbiol., 64, 1477, 10.1128/AEM.64.4.1477-1483.1998 Shourideh, 2012, Effects of D-tagatose and inulin on some physicochemical, rheological and sensory properties of dark chocolate, Int. J. Biosci. Biochem. Bioinf., 314 Sik, 2021, Exploring the rosmarinic acid profile of dark chocolate fortified with freeze-dried lemon balm extract using conventional and non-conventional extraction techniques, LWT (Lebensm.-Wiss. & Technol.), 147 Slavin, 2013, Fiber and prebiotics: mechanisms and health benefits, Nutrients, 5, 1417, 10.3390/nu5041417 Spencer, 2008, Food for thought: the role of dietary flavonoids in enhancing human memory, learning and neuro-cognitive performance, Proc. Nutr. Soc., 67, 238, 10.1017/S0029665108007088 Spencer, 2009, Flavonoids and brain health: multiple effects underpinned by common mechanisms, Genes and Nutrition, 4, 243, 10.1007/s12263-009-0136-3 Sumiyoshi, 2019, Sub-chronic consumption of dark chocolate enhances cognitive function and releases nerve growth factors: a parallel-group randomized trial, Nutrients, 11, 1, 10.3390/nu11112800 Syafiq, 2014, Mixture experiment on rheological properties of dark chocolate as influenced by cocoa butter substitution with xanthan gum/corn starch/glycerin blends, International Food Research Journal, 21, 1887 Taslim, 2020, Effect of chocolate soybean drink on nutritional status, gamma interferon, vitamin D, and calcium in newly lung tuberculosis patients, Open Access Macedonian Journal of Medical Sciences, 8, 210, 10.3889/oamjms.2020.5233 Thorsen, 2009, Food and nutrition, Information Resources in Toxicology, 43, 281, 10.1016/B978-0-12-373593-5.00031-8 Tokede, 2011, Effects of cocoa products/dark chocolate on serum lipids: a meta-analysis, Eur. J. Clin. Nutr., 65, 879, 10.1038/ejcn.2011.64 Toker, 2018, Formulation of dark chocolate as a carrier to deliver eicosapentaenoic and docosahexaenoic acids: effects on product quality, Food Chem., 254, 224, 10.1016/j.foodchem.2018.02.019 Toker, 2018, Formulation of dark chocolate as a carrier to deliver eicosapentaenoic and docosahexaenoic acids: effects on product quality, Food Chem., 254, 224, 10.1016/j.foodchem.2018.02.019 Toker, 2019, Chocolate quality and conching, Trends Food Sci. Technol., 91, 446, 10.1016/j.tifs.2019.07.047 Toplar, 2017, vols. 5–42 Trautwein, 2007, Phytosterols: natural compounds with established and emerging health benefits, OCL - Oleagineux Corps Gras Lipides, 14, 259, 10.1051/ocl.2007.0145 Triebel, 2012, Modulation of inflammatory gene expression by a bilberry (Vaccinium myrtillus L.) extract and single anthocyanins considering their limited stability under cell culture conditions, J. Agric. Food Chem., 60, 8902, 10.1021/jf3028842 Tsao, 2010, Chemistry and biochemistry of dietary polyphenols, Nutrients, 2, 1231, 10.3390/nu2121231 Urbańska, 2019, Comparison of the total polyphenol content and antioxidant activity of chocolate obtained from roasted and unroasted cocoa beans from different regions of the world, Antioxidants, 8, 10.3390/antiox8080283 Verbeke, 2015, Towards microbial fermentation metabolites as markers for health benefits of prebiotics, Nutr. Res. Rev., 28, 42, 10.1017/S0954422415000037 Vickers, 2017, Animal communication: when I'm calling you, will you answer too?, Curr. Biol., 27, R713, 10.1016/j.cub.2017.05.064 Virgens, 2021, Relationship between bioactive compounds and sensory properties of dark chocolate produced from Brazilian hybrid cocoa, Int. J. Food Sci. Technol., 56, 1905, 10.1111/ijfs.14820 Vita, 2005, Polyphenols and cardiovascular disease: effects on endothelial and platelet function, Am. J. Clin. Nutr., 81, 292, 10.1093/ajcn/81.1.292S Waghmode, 2020, Probiotic sugar confectionery fortified with flax seeds (Linum usitatissimum L.), J. Food Sci. Technol., 57, 1964, 10.1007/s13197-020-04276-x Wan, 2001, Effects of cocoa powder and dark chocolate on LDL oxidative susceptibility and prostaglandin concentrations in humans, Am. J. Clin. Nutr., 74, 596, 10.1093/ajcn/74.5.596 Wang-Polagruto, 2006, Chronic consumption of flavanol-rich cocoa improves endothelial function and decreases vascular cell adhesion molecule in hypercholesterolemic postmenopausal women, J. Cardiovasc. Pharmacol., 47 Weisel, 2006, An anthocyanin/polyphenolic-rich fruit juice reduces oxidative DNA damage and increases glutathione level in healthy probands, Biotechnol. J., 1, 388, 10.1002/biot.200600004 Wong, 2018 Xie, 2016, Bioavailability of anthocyanins and colonic polyphenol metabolites following consumption of aronia berry extract, Food Chem., 211, 860, 10.1016/j.foodchem.2016.05.122 Zhang, 2019, Health benefits of dietary marine DHA/EPA-enriched glycerophospholipids, Prog. Lipid Res., 75 Zugravu, 2019, Dark chocolate: to eat or not to eat? A review, J. AOAC Int., 102, 1388, 10.5740/jaoacint.19-0132