DNA studies of a Chesapeake Bay population of the tunicate Molgula manhattensis (DeKay) (Ascidiacea)
Tóm tắt
Gonadal development and total gonadal DNA of M. manhattensis were studied. Evidence of atrophication and of phagocytosis of residual gametes was found during histological examination. Colorimetric estimations of gonadal DNA indicated that at least two spawning peaks occurred during the summer and suggested that egg release was suppressed by water temperatures above 20 C.
Tài liệu tham khảo
Atkin, N. B., G. Mattinson, W. Becak, and S. Ohno. 1965. The comparative DNA content of 19 species of placental mammals, reptiles, and birds. Chromosoma 17:1–10.
—, and S. Ohno. 1967. DNA values of four primitive chordates. Chromosoma 23:10–13.
Burton, K. 1956. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J. 62:315–323.
Carlisle, D. B. 1951. Corpora lutea in an ascidian, Ciona intestinalis, Quart. J. Micr. Sci. 92:201–203.
Colombera, D., and M. Sala. 1972. The male chromosomes of Diplosoma listerianum (Ascidiacea). Caryologia 25:409–415.
Costello, D. P., M. E. Davidson, A. Eggers, M. H. Fox, and C. Henley. 1957. Methods for obtaining and handling marine eggs and embryos. Lancaster Press, Inc. Lancaster, Penna. 247 p.
Crampton, H. E. 1899. Studies on the early history of the ascidian egg. I. The ovarian history of the egg of Molgula manhattensis. J. Morph. (supplement) 15:29–56.
Dybern, B. I. 1965. The life cycle of Ciona intestinalis (L.) f. typica in relation to the environmental temperature. Oikos 16:109–131.
Grave, B. H. 1933. Rate of growth, age at sexual maturity, and duration of life of certain sessile organisms at Woods Hole, Massachusetts. Biol. Bull. 65:375–386.
Gray, P. 1964. Handbook of basic microtechnique. McGraw-Hill Book Comp. N. Y. 302 pp.
Grosch, D. S. 1958. Feulgen-positive cytoplasm of Molgula eggs. Nature 181:1078.
Harvey, L. A. 1927. The history of the cytoplasmic inclusion of the egg of Ciona intestinalis (L.) during oogenesis and fertilisation. Proc. Roy. Soc. Lond., ser. B. 101:136–162.
Millar, R. H. 1952. The annual growth and reproductive cycle in four ascidians. J. Mar. Biol. Ass. U.K. 31:41–61.
— 1954. The annual growth and reproductive cycle of the ascidian Dendrodoa grossularia (Van Beneden). J. Mar. Biol. Ass. U.K. 33:33–48.
—. 1971. The biology of ascidians. Adv. Mar. Biol. 9:1–100.
Ohno, S., and N. B. Atkin. 1966. Comparative DNA values and chromosome complements of 8 species of fishes. Chromosoma 18:455–466.
Orton, J. H. 1920. Sea-temperature, breeding, and distribution in marine animals. J. Mar. Biol. Ass. U.K. 12:339–366.
Penman, S. 1969. Preparation of purified nuclei and nucleoli from mammalian cells. p. 35–48. In K. Habel and N. P. Salzman (ed.) Fundamental techniques in virology. Acad. Press, N. Y.
Pérés, J. M. 1952. Recherches sur le cycle sexuel de Ciona intestinalis (L.). Archiv. Anat. micr. Morph. Exp. T. 41:153–183. In Millar, R. H. 1954. The annual growth and reproductive cycle of the ascidian Dendrodoa grossularia (Van Beneden). J. Mar. Biol. Ass. U.K. 33:33–48.
Sabbadin, A. 1957. Il ciclo biologico di Ciona intestinalis, Molgula manhattensis e Styeia plicata nella Laguna Veneta. Arch Oc. e. Limnol. 11:1–28.
Shatkin, A. J. 1969. Colorimetric reaction for DNA, RNA, and protein determination, p. 231–237. In K. Habel and N. P. Salzman (ed.) Fundamental techniques in virology. Acad. Press, N. Y.
Taylor, K. M. 1967. The chromosomes of some lower chordates. Chromosoma 21:181–188.