DNA sequencing confirms PCR-RFLP identification of wild caught Larroussius sand flies from Crete and Cyprus

Absavaran, 2009, Identification of sand flies of the subgenus Larroussius based on molecular and morphological characters in North Western Iran, Iran. J. Arthropod Borne Dis., 3, 22 Alten, 2016, Seasonal dynamics of phlebotomine sand fly species proven vectors of mediterranean leishmaniasis caused by Leishmania infantum, PLoS Negl. Trop. Dis., 10, e0004458, 10.1371/journal.pntd.0004458 Antoniou, 2008, Leishmania donovani leishmaniasis in Cyprus, Lancet Infect. Dis., 8, 6, 10.1016/S1473-3099(07)70297-9 Antoniou, 2009, Increasing incidence of zoonotic visceral leishmaniasis on Crete, Greece, Emerg. Infect. Dis., 15, 932, 10.3201/eid1506.071666 Antoniou, 2013, The role of indigenous phlebotomine sandflies and mammals in the spreading of leishmaniasis agents in the Mediterranean region, Euro Surveill., 18, 20540, 10.2807/1560-7917.ES2013.18.30.20540 Aransay, 2000, Phylogenetic relationships of phlebotomine sandflies inferred from small subunit nuclear ribosomal DNA, Insect Mol. Biol., 9, 157, 10.1046/j.1365-2583.2000.00168.x Bettini, 1986, Leishmaniasis in sardinia: II. Natural infection of Phlebotomus perniciosus Newstead, 1911, by Leishmania infantum Nicolle, 1908, in the province of Cagliari, Trans. R. Soc. Trop. Med. Hyg., 80, 458, 10.1016/0035-9203(86)90344-5 Bounamous, 2014, Limits of a rapid identification of common Mediterranean sandflies using polymerase chain reaction-restriction fragment length polymorphism, Mem. Inst. Oswaldo Cruz, 109, 466, 10.1590/0074-0276130584 Chaskopoulou, 2016, Species composition, activity patterns and blood meal analysis of sand fly populations (Diptera: psychodidae) in the metropolitan region of Thessaloniki, an endemic focus of canine leishmaniasis, Acta Trop., 158, 170, 10.1016/j.actatropica.2016.03.006 Christodoulou, 2012, Re-emergence of visceral and cutaneous leishmaniasis in the Greek Island of Crete, Vector Borne Zoonotic Dis., 12, 214, 10.1089/vbz.2011.0004 Depaquit, 2013, A taxonomic study of Phlebotomus (Larroussius) perfiliewi s.l, Infect. Genet. Evol., 20, 500, 10.1016/j.meegid.2013.10.006 Depaquit, 2015, Mitochondrial DNA intraspecific variability in Sergentomyia minuta (Diptera: psychodidae), J. Med. Entomol., 52, 819, 10.1093/jme/tjv075 Depaquit, 2014, Molecular systematics applied to Phlebotomine sandflies: review and perspectives, Infect. Genet. Evol., 28, 744, 10.1016/j.meegid.2014.10.027 Di Muccio, 2000, Phylogenetic analysis of Phlebotomus species belonging to the subgenus Larroussius (Diptera, psychodidae) by ITS2 rDNA sequences, Insect Biochem. Mol. Biol., 30, 387, 10.1016/S0965-1748(00)00012-6 Dostálová, 2012, Leishmania development in sand flies: parasite-vector interactions overview, Parasites Vectors, 5, 276, 10.1186/1756-3305-5-276 Ergunay, 2012, Molecular evidence indicates that Phlebotomus major sensu lato (Diptera: psychodidae) is the vector species of the recently-identified sandfly fever Sicilian virus variant: sandfly fever turkey virus, Vector Borne Zoonotic Dis., 12, 690, 10.1089/vbz.2011.0927 Esseghir, 2000, Speciation of Phlebotomus sandflies of the subgenus Larroussius coincided with the late Miocene-Pliocene aridification of the Mediterranean subregion, Biol. J. Linn. Soc., 70, 189, 10.1111/j.1095-8312.2000.tb00207.x Folmer, 1994, DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates, Mol. Mar. Biol. Biotechnol., 3, 294 Fujita, 2012, Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic, Acta Trop., 121, 93, 10.1016/j.actatropica.2011.10.004 Hebert, 2003, Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species, Proc. Biol. Sci., 270, S96, 10.1098/rsbl.2003.0025 Huelsenbeck, 2001, MRBAYES: Bayesian inference of phylogenetic trees, Bioinformatics, 17, 754, 10.1093/bioinformatics/17.8.754 Hughes, 2014, Discrimination of the Plasmodium mexicanum vectors Lutzomyia stewarti and Lutzomyia vexator by a PCR-RFLP assay and Wolbachia infection, J. Vector Ecol., 39, 224, 10.1111/j.1948-7134.2014.12092.x Ivović, 2007, Faunistic study of sandflies in Greece, Med. Vet. Entomol., 21, 121, 10.1111/j.1365-2915.2006.00649.x Jinbo, 2011, Current progress in DNA barcoding and future implications for entomology, Entomol. Sci., 14, 107, 10.1111/j.1479-8298.2011.00449.x Killick-Kendrick, 1990, Phlebotomine vectors of the leishmaniases: a review, Med. Vet. Entomol., 4, 1, 10.1111/j.1365-2915.1990.tb00255.x Killick-Kendrick, 1999, The biology and control of phlebotomine sand flies, Clin. Dermatol., 17, 279, 10.1016/S0738-081X(99)00046-2 Koliou, 2014, A cluster of four cases of cutaneous leishmaniasis by Leishmania donovani in Cyprus: a case series, J. Med. Case Rep., 8, 354, 10.1186/1752-1947-8-354 Krüger, 2011, The sandflies (Diptera: psychodidae, Phlebotominae) in military camps in Northern Afghanistan (2007–2009), as identified by morphology and DNA barcoding, Ann. Trop. Med. Parasitol., 105, 163, 10.1179/136485911X12899838683241 Kumar, 2012, DNA barcoding for identification of sand flies (Diptera: psychodidae) in India, Mol. Ecol. Resour., 12, 414, 10.1111/j.1755-0998.2012.03117.x Léger, 1988, Isolation and typing of Leishmania infantum from Phlebotomus neglectus on the island of Corfu, Greece, Trans. R. Soc. Trop. Med. Hyg., 82, 419, 10.1016/0035-9203(88)90145-9 Léger, 2000, Phlebotomine sandflies (Diptera-Psychodidae) of the isle of Cyprus. II-isolation and typing of leishmania (Leishmania) infantum Nicolle, 1908 (zymodeme MON 1) from Phlebotomus (Larroussius) tobbi Adler and Theodor, 1930, Parasite, 7, 143, 10.1051/parasite/2000072143 Lanfear, 2012, Partitionfinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses, Mol. Biol. Evol., 29, 1695, 10.1093/molbev/mss020 Latrofa, 2011, Multilocus molecular and phylogenetic analysis of phlebotomine sand flies (Diptera: psychodidae) from Southern Italy, Acta Trop., 119, 91, 10.1016/j.actatropica.2011.04.013 Latrofa, 2012, Towards a rapid molecular identification of the common phlebotomine sand flies in the Mediterranean region, Vet. Parasitol., 184, 267, 10.1016/j.vetpar.2011.08.031 Lewis, 1978, Phlebotomid sand flies (Diptera: psychodidae) of the oriental region, Bull. World Health Organ., 37, 1 Lewis, 1982, A taxonomic review of the genus Phlebotomus (Diptera: psychodidae), Bull. Br. Mus. Nat. Hist. Entomol., 45, 121 Llanes-Acevedo, 2016, DNA sequence analysis suggests that cytb-nd1 PCR-RFLP may not be applicable to sandfly species identification throughout the Mediterranean region, Parasitol. Res., 115, 1287, 10.1007/s00436-015-4865-5 Maia, 2015, Exploring the utility of phylogenetic analysis of cytochrome oxidase gene subunit I as a complementary tool to classical taxonomical identification of phlebotomine sand fly species (Diptera, Psychodidae) from Southern Europe, Acta Trop., 144, 1, 10.1016/j.actatropica.2014.12.013 Maroli, 1987, Natural infection of Phlebotomus perfiliewi with Leishmania infantum in a cutaneous leishmaniasis focus of the Abruzzi region, Italy, Trans. R. Soc. Trop. Med. Hyg., 81, 596, 10.1016/0035-9203(87)90420-2 Maroli, 2013, Phlebotomine sandflies and the spreading of leishmaniases and other diseases of public health concern, Med. Vet. Entomol., 27, 123, 10.1111/j.1365-2915.2012.01034.x Mazeris, 2010, Leishmaniases and the Cyprus paradox, Am. J. Trop. Med. Hyg., 82, 441, 10.4269/ajtmh.2010.09-0282 Merino-Espinosa, 2016, Differential ecological traits of two Phlebotomus sergenti mitochondrial lineages in Southwestern Europe and their epidemiological implications, Trop. Med. Int. Health, 10.1111/tmi.12686 Minter, 2013, Molecular identification of sand flies (Diptera: psychodidae) in Eastern North America by using PCR-RFLP, J. Med. Entomol., 50, 920, 10.1603/ME12014 Ntais, 2013, Leishmaniases in Greece, Am. J. Trop. Med. Hyg., 89, 906, 10.4269/ajtmh.13-0070 Ntais, 2014, Will the introduction of Leishmania tropica MON-58, in the island of Crete, lead to the settlement and spread of this rare zymodeme?, Acta Trop., 132, 125, 10.1016/j.actatropica.2014.01.003 Parrot, 1930, Notes sur les Phlébotomes IV. Phlebotomus perfiliewi n. sp, Arch. Inst. Pasteur Algér., 8, 303 de S. Pinto, 2015, DNA barcoding of neotropical sand flies (Diptera, psychodidae, phlebotominae): species identification and discovery within Brazil, PLoS One, 10, e0140636, 10.1371/journal.pone.0140636 Rioux, 1979, Ecology of leishmaniasis in the south of France. 11. Canine leishmaniasis: successful experimental transmission from dog to dog by the bite of Phlebotomus ariasi Tonnoir, 1921, Ann. Parasitol. Hum. Comp., 54, 401, 10.1051/parasite/1979544401 Ronquist, 2012, MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space, Syst. Biol., 61, 539, 10.1093/sysbio/sys029 Seblova, 2015, Natural hybrid of Leishmania infantum/L. donovani: development in Phlebotomus tobbi, P. perniciosus and Lutzomyia longipalpis and comparison with non-hybrid strains differing in tissue tropism, Parasite Vectors, 8 Silvestro, 2011, raxmlGUI: a graphical front-end for RAxML, Org. Divers. Evol., 12, 335, 10.1007/s13127-011-0056-0 Stamatakis, 2014, RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies, Bioinformatics, 30, 1312, 10.1093/bioinformatics/btu033 Svobodová, 2009, Cutaneous leishmaniasis caused by Leishmania infantum transmitted by Phlebotomus tobbi, Int. J. Parasitol., 39, 251, 10.1016/j.ijpara.2008.06.016 Tamura, 2013, MEGA6: molecular evolutionary genetics analysis version 6.0, Mol. Biol. Evol., 30, 2725, 10.1093/molbev/mst197 Terayama, 2008, Molecular typing of sand fly species (Diptera, Psychodidae, Phlebotominae) from areas endemic for Leishmaniasis in Ecuador by PCR-RFLP of 18S ribosomal RNA gene, J. Vet. Med. Sci., 70, 907, 10.1292/jvms.70.907 Thompson, 1994, CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice, Nucleic Acids Res., 22, 4673, 10.1093/nar/22.22.4673 Tiwary, 2012, PCR-RFLP based method for molecular differentiation of sand fly species Phlebotomus argentipes, Phlebotomus papatasi, and Sergentomyia babu found in India, J. Med. Entomol., 49, 1515, 10.1603/ME12033 World Health Organization, 2010. Control of the leishmaniases. World Health Organ Tech Rep Ser xii–xiii, 1–186, back cover. Yang, 2006