D1 and D2 Receptor Gene Expression in the Rat Frontal Cortex: Cellular Localization in Different Classes of Efferent Neurons

European Journal of Neuroscience - Tập 7 Số 5 - Trang 1050-1063 - 1995
Patrícia Gaspar1, Bertrand Bloch2, Catherine Le Moine2
1INSERM U106, Bâtiment de Pédiatrie, Hôpital Salpêtrière, Paris France
2EP CNRS 74, Laboratoire d'Histologie‐Embryologie, Universite de Bordeaux II, Bâtiment 38, zone Nord, 146 rue Leo Saignat, 33076 Bordeaux Cedex, France

Tóm tắt

Abstract

The dopaminergic input to the frontal cortex has an important role in motor and cognitive functions. These effects are mediated by dopamine receptors both of type D1 and of type D2, although the neural circuits involved are not completely understood. We used in situ hybridization to determine the cellular localization of D1 and D2 receptor mRNAs in the rat frontal cortex. Retrograde tracing was used in the same animals to identify the main cortical efferent populations. Fluorogold was injected into the different cortical targets of the frontal cortex and sections were hybridized with D1 and D2 35S‐labelled cRNA probes. D1 and D2 mRNA‐containing neurons were present in all the cortical areas investigated, with greater expression in the medial prefrontal, insular and cingulate cortexes and lower expression in the motor and parietal cortexes. Neurons containing D1 mRNA were most abundant in layer Vlb; they were also present in layers Vla and V of all cortical layers and in layer II of the medial prefrontal, cingulate and insular areas. Double labelling with fluorogold demonstrated that D1 mRNA was present in corticocortical, corticothalamic and corticostriatal neurons. Neurons containing D2 mRNA were essentially restricted to layer V, but only in corticostriatal and corticocortical neurons. Neither D1 nor D2 mRNA was found in corticospinal or corticopontine neurons. The present results demonstrate that D1 and D2 receptor genes are expressed in efferent cortical populations, with higher expression for D1. In spite of an overlap in some cortical layers, the expression of D1 and D2 receptor genes is specific for different categories of pyramidal neurons.

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Tài liệu tham khảo

10.1016/0006-8993(92)90420-E

10.1016/0006-8993(93)90857-J

10.1016/0166-2236(91)90179-X

Berger B., 1994, Phylogeny and Ontogeny uf Catecholamine Systems in the CNS of Vertebrates, 293

10.1016/0306-4522(87)90008-X

10.1016/0006-8993(91)91456-B

10.1002/cne.902960407

10.1016/0306-4522(87)90038-8

10.1016/0306-4522(86)90010-2

10.1016/0006-8993(87)90450-1

10.1073/pnas.88.9.3772

10.1016/0006-8993(91)90933-M

10.1002/ana.410300308

10.1126/science.2799392

10.1113/jphysiol.2006.113050

10.1016/0306-4522(84)90059-9

10.1073/pnas.86.22.9015

10.1016/0306-4522(91)90196-U

10.1073/pnas.89.24.11988

10.1016/0169-328X(92)90107-M

10.1152/jn.1993.69.2.416

10.1073/pnas.87.1.230

Moine C., 1995, D1 and D2 dopamine receptor gene expression in the rat striatum: sensitive cRNA probes demonstrate prominent segregation of D1 and D2 mRNAs in neuronal populations of the dorsal and ventral striatum, J. Comp. Neurol.

10.1073/pnas.88.10.4205

10.1073/pnas.90.19.8861

10.1523/JNEUROSCI.10-08-02587.1990

10.1016/0306-4522(88)90285-0

10.1038/342926a0

10.1073/pnas.87.17.6723

10.1016/0006-8993(91)91190-C

10.1016/0006-8993(87)90509-9

10.1016/0306-4522(92)90362-6

10.1111/j.1460-9568.1993.tb00540.x

10.1016/0304-3940(88)90779-3

10.1016/0306-4522(91)90296-Z

10.1002/cne.902860402

10.1126/science.1825731

10.1016/1044-5765(92)90011-P

10.1016/0165-6147(94)90323-9

10.1002/cne.902900205

10.1523/JNEUROSCI.14-01-00088.1994

10.1016/0006-8993(88)91427-8

10.1016/0006-8993(90)90777-9

10.1073/pnas.91.12.5720

Streit P., 1984, Cerebral Cortex Vol. II. Funcrional Properties of Cortical Cells, 119

10.1016/0166-2236(93)90103-S

Thieny A. M., 1994, Moror and Cognitive Functions of the Prefrontal Cortex, 35

10.1111/j.1460-9568.1992.tb00111.x

10.1111/j.1460-9568.1990.tb00008.x

10.1111/j.1460-9568.1991.tb00036.x

10.1523/JNEUROSCI.13-06-02551.1993

10.1073/pnas.88.5.1859

10.1016/0361-9230(93)90075-M

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Thông tin xuất bản

European Journal of Neuroscience

Tập 7 Số 5

1050-1063

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