Cytopathic Effects of the Major Surface Protein and the Chymotrypsinlike Protease of<i>Treponema denticola</i>

Infection and Immunity - Tập 66 Số 5 - Trang 1869-1877 - 1998
J. Christopher Fenno1, Pauline M. Hannam1, WK Leung2, Muneaki Tamura1, VELI‐JUKKA UITTO2, B C McBride2
1<!--label omitted: 1-->Department of Microbiology and Immunology1 and
2<!--label omitted: 2-->Department of Oral Biological and Medical Sciences,2 University of British Columbia, Vancouver, Canada

Tóm tắt

ABSTRACTProminent antigens ofTreponema denticolahave been suggested to be mediators of the cytopathic effects typically seen in periodontal disease. In the present study of theT. denticolamajor surface protein (Msp) and the surface-expressed chymotrypsinlike protease complex (CTLP), we characterized the ability of these proteins to adhere to and lyse epithelial cells. Msp and CTLP were closely associated in spirochete outer membranes. Purified Msp, both native and recombinant, and CTLP bound to glutaraldehyde-fixed periodontal ligament epithelial cells. Adherence of Msp was partially blocked by specific antibodies. Adherence of CTLP was partially blocked by serine protease inhibitors and was further inhibited by specific antibodies. Both native Msp and CTLP were cytotoxic toward periodontal ligament epithelial cells, and their cytotoxicity was inhibited by the same treatments that inhibited adherence. Msp, but not CTLP, lysed erythrocytes. Msp complex (partially purified outer membranes free of protease activity) was cytotoxic toward a variety of different cell types. Pore-forming activities of recombinant Msp in black lipid model membrane assays and in HeLa cell membranes were similar to those reported for the native protein, supporting the hypothesis that Msp cytotoxicity was due to its pore-forming activity.

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Infection and Immunity

Tập 66 Số 5

1869-1877

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