Cultured epithelial cells response to phototherapy with low intensity laser

Lasers in Surgery and Medicine - Tập 39 Số 4 - Trang 365-372 - 2007
Fernanda de Paula Eduardo1, Dolores Úrsula Mehnert2, Telma Alves Monezi2, Denise Maria Zezéll3, Mark M. Schubert4, Carlos Eduardo5, Márcia Martins Marques5
1Department of Oral Diagnosis, School of Dentistry, University of São Paulo, São Paulo, Brazil
2Department of Microbiology, Institute of Biological Sciences, University of São Paulo, São Paulo, Brazil
3Centro de Lasers e Aplicações, Instituto de Pesquisas Energéticas e Nucleares, São Paulo, São Paulo, Brazil
4Department of Oral Medicine, University of Washington, Oral Medicine Service, Seattle Cancer Care Alliance, Seattle, Washington
5Department of Restorative Dentistry, School of Dentistry, University of São Paulo, Sao Pãulo, Brazil

Tóm tắt

AbstractBackground and ObjectivesLittle is known about the intracellular response of epithelial cells to phototherapy. The aim of this in vitro study was to analyze the effect of phototherapy with low‐energy lasers with different wavelengths and powers on cultured epithelial cell growth under different nutritional conditions.Study Design/Materials and MethodsEpithelial cell cultures (Vero cell line) grown in nutritional deficit in culture medium supplemented with 2% fetal bovine serum (FBS) were irradiated with low‐energy laser from one to three times with a GaAlAs laser (660 nm) and InGaAlP (780 nm), 40 and 70 mW, respectively, with 3 or 5 J/cm2. Cell growth was indirectly assessed by measuring the cell mitochondrial activity.ResultsNonirradiated cell cultures grown in nutritional regular medium supplemented with 10% FBS produced higher cell growth than all cultures grown in nutritional deficit irradiated or not. The overall cell growth of cultures grown under nutritionally deficit conditions was significantly improved especially when irradiated with 780 nm for three times.ConclusionsPhototherapy with the laser parameters tested increases epithelial cell growth rate for cells stressed by growth under nutritionally deficient states. This cell growth improvement is directly proportional to the number of irradiations; however, was not enough to reach the full cell growth potential rate of Vero epithelial cell line observed when growing under nutritional regular condition. Lasers Surg. Med. 39: 365–372, 2007. © 2007 Wiley‐Liss, Inc.

Từ khóa


Tài liệu tham khảo

10.1002/lsm.1900050105

10.1002/lsm.1107

10.1002/lsm.10107

10.1002/lsm.20008

10.1007/s10103-006-0379-9

10.1002/lsm.20298

10.1111/j.1524-4725.2005.31086

10.1002/lsm.1900120512

10.1016/1011-1344(92)85032-P

10.1089/pho.2005.23.161

10.1002/(SICI)1096-9101(1996)18:2<171::AID-LSM7>3.0.CO;2-P

10.1002/lsm.20148

10.1046/j.1523-1747.1999.00684.x

10.1016/j.jphotobiol.2004.03.002

10.1002/lsm.1900140408

10.1016/1011-1344(91)80271-I

10.1016/S1011-1344(96)07426-X

Thông tin
Thông tin xuất bản

Lasers in Surgery and Medicine

Tập 39 Số 4

365-372

Thông tin tác giả