Crystal structure of FabZ-ACP complex reveals a dynamic seesaw-like catalytic mechanism of dehydratase in fatty acid biosynthesis

Cell Research - Tập 26 Số 12 - Trang 1330-1344 - 2016
Lin Zhang1, Jianfeng Xiao2, Jianrong Xu1, Tianran Fu1, Zhiwei Cao1, Liang Zhu1, Hongzhuan Chen3, Xu Shen2, Hualiang Jiang2, Liang Zhang3
1Department of Pharmacology and Chemical Biology, Shanghai Jiao Tong University School of Medicine, Shanghai, China
2State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
3Shanghai Universities Collaborative Innovation Center for Translational Medicine, Shanghai, China

Tóm tắt

Từ khóa


Tài liệu tham khảo

Smith S, Witkowski A, Joshi AK . Structural and functional organization of the animal fatty acid synthase. Prog Lipid Res 2003; 42:289–317.

White SW, Zheng J, Zhang YM . Rock. The structural biology of type II fatty acid biosynthesis. Annu Rev Biochem 2005; 74:791–831.

Cronan JE, Thomas J . Bacterial fatty acid synthesis and its relationships with polyketide synthetic pathways. Methods Enzymol 2009; 459:395–433.

Anghel SI, Wahli W . Fat poetry: a kingdom for PPAR gamma. Cell Res 2007; 17:486–511.

Clay HB, Parl AK, Mitchell SL, Singh L, Bell LN, Murdock DG . Altering the mitochondrial fatty acid synthesis (mtFASII) pathway modulates cellular metabolic states and bioactive lipid profiles as revealed by metabolomic profiling. PLoS One 2016; 11:e0151171.

Wu J, Sun Y, Zhao Y, et al. Deficient plastidic fatty acid synthesis triggers cell death by modulating mitochondrial reactive oxygen species. Cell Res 2015; 25:621–633.

Ronnett GV, Kim EK, Landree LE, Tu Y . Fatty acid metabolism as a target for obesity treatment. Physiol Behav 2005; 85:25–35.

Cooke AA, Connaughton RM, Lyons CL, McMorrow AM, Roche HM . Fatty acids and chronic low grade inflammation associated with obesity and the metabolic syndrome. Eur J Pharmacol 2016; 785:207–214.

Taylor EM, Jones AD, Henagan TM . A review of mitochondrial-derived fatty acids in epigenetic regulation of obesity and type 2 diabetes. J Nutrit Health Food Sci 2014; 2:1–4.

Zhang Y, Kent JW, Lee A, et al. Fatty acid binding protein 3 (fabp3) is associated with insulin, lipids and cardiovascular phenotypes of the metabolic syndrome through epigenetic modifications in a Northern European family population. BMC Med Genomics 2013, 6:9–22.

Arumugam S, Sreedhar R, Thandavarayan RA, Karuppagounder V, Watanabe K . Targeting fatty acid metabolism in heart failure: is it a suitable therapeutic approach. Drug Discov Today 2016; 21:1003–1008.

Kuhajda FP . Fatty acid synthase and cancer: new application of an old pathway. Cancer Res 2006; 66:5977–5980.

Menendez JA, Lupu R . Fatty acid synthase and the lipogenic phenotype in cancer pathogenesis. Nat Rev Cancer 2007; 7:763–777.

Rossi S, Graner E, Febbo P, et al. Fatty acid synthase expression defines distinct molecular signatures in prostate cancer. Mol Cancer Res 2003; 1:707–715.

Migita T, Ruiz S, Fornari A, et al. Fatty acid synthase: a metabolic enzyme and candidate oncogene in prostate cancer. J Natl Cancer Inst 2009; 101:519–532.

Vazquez-Martin A, Colomer R, Brunet J, Lupu R, Menendez JA . Overexpression of fatty acid synthase gene activates HER1/HER2 tyrosine kinase receptors in human breast epithelial cells. Cell Prolif 2008; 41:59–85.

Jin Q, Yuan LX, Boulbes D, et al. Fatty acid synthase phosphorylation: a novel therapeutic target in HER2-overexpressing breast cancer cells. Breast Cancer Res 2010; 12:R96–R110.

Jenke-Kodama H, Sandmann A, Müller R, Dittmann E . Evolutionary implications of bacterial polyketide synthases. Mol Biol Evol 2005; 22:2027–2039.

Maier T, Jenni S, Ban N . Architecture of mammalian fatty acid synthase at 4.5 A resolution. Science 2006; 311:1258–1262.

Jenni S, Leibundgut M, Maier T, Ban N . Architecture of a fungal fatty acid synthase at 5 A resolution. Science 2006; 311:1263–1267.

Lomakin IB, Xiong Y, Steitz TA . The crystal structure of yeast fatty acid synthase, a cellular machine with eight active sites working together. Cell 2007; 129:319–332.

Jenni S, Leibundgut M, Boehringer D, Frick C, Mikolásek B, Ban N . Structure of fungal fatty acid synthase and implications for iterative substrate shuttling. Science 2007; 316:254–261.

Maier T, Leibundgut M, Ban N . The crystal structure of a mammalian fatty acid synthase. Science 2008; 321:1315–1322.

Cronan JE, Thomas J . Bacterial fatty acid synthesis and its relationships with polyketide synthetic pathways. Methods Enzymol 2009; 459:395–433.

Rafi S, Novichenok P, Kolappan S, et al. Structure of acyl carrier protein bound to FabI, the FASII enoyl reductase from Escherichia coli. J Biol Chem 2006; 281:39285–39293.

Zhang L, Liu W, Hu T, et al. Structural basis for catalytic and inhibitory mechanisms of beta-hydroxyacyl-acyl carrier protein dehydratase (FabZ). J Biol Chem 2008; 283:5370–5379.

Kimber MS, Martin F, Lu Y, et al. The structure of (3R)-hydroxyacyl-acyl carrier protein dehydratase (FabZ) from Pseudomonas aeruginosa. J Biol Chem 2004; 279:52593–52602.

Kostrewa D, Winkler FK, Folkers G, Scapozza L, Perozzo R . The crystal structure of PfFabZ, the unique beta-hydroxyacyl-ACP dehydratase involved in fatty acid biosynthesis of Plasmodium falciparum. Protein Sci 2005; 14:1570–1580.

Nguyen C, Haushalter RW, Lee DJ, et al. Trapping the dynamic acyl carrier protein in fatty acid biosynthesis. Nature 2014; 505:427–431.

Zeng D, Zhao J, Chung HS, Guan Z, Raetz CR, Zhou P . Mutants resistant to LpxC inhibitors by rebalancing cellular homeostasis. J Biol Chem 2013; 288:5475–5486.

Heath RJ, White SW, Rock CO . Lipid biosynthesis as a target for antibacterial agents. Prog Lipid Res 2001; 40:467–497.

Campbell JW, Cronan JE Jr . Bacterial fatty acid biosynthesis: targets for antibacterial drug discovery. Annu Rev Microbiol 2001; 55:305–332.

Orita H, Coulter J, Lemmon C, et al. Selective inhibition of fatty acid synthase for lung cancer treatment. Clin Cancer Res 2007; 13:7139–7145.

He L, Zhang L, Liu X, et al. Discovering potent inhibitors against the beta-hydroxyacyl-acyl carrier protein dehydratase (FabZ) of Helicobacter pylori: structure-based design, synthesis, bioassay, and crystal structure determination. J Med Chem 2009; 52:2465–2481.

Hardwicke MA, Rendina AR, Williams SP, et al. A human fatty acid synthase inhibitor binds β-ketoacyl reductase in the keto-substrate site. Nat Chem Biol 2014; 10:774–779.

Liu W, Luo C, Han C, et al. A new beta-hydroxyacyl-acyl carrier protein dehydratase (FabZ) from Helicobacter pylori: molecular cloning, enzymatic characterization, and structural modeling. Biochem Biophys Res Commun 2005; 333:1078–1086.

Liu W, Du L, Zhang L, Chen J, Shen X, Jiang H . Helicobacter pylori acyl carrier protein: expression, purification, and its interaction with beta-hydroxyacyl-ACP dehydratase. Protein Expr Purif 2007; 52:74–81.

Pasta S, Witkowski A, Joshi AK, Smith S . Catalytic residues are shared between two pseudosubunits of the dehydratase domain of the animal fatty acid synthase. Chem Biol 2007; 14:1377–1385.

Jones G, Willett P, Glen RC, Leach AR, Taylor R . Development and validation of a genetic algorithm for flexible docking. J Mol Biol 1997; 267:727–748.

Willemsen MJ, André T, Wanner R, et al. Microscale thermophoresis: interaction analysis and beyond. J Mol Struct 2014; 1077:101–113.

Maximino P, Horta PM, Santos LC, Oliveira CL, Fisberg M . Fatty acid intake and metabolic syndrome among overweight and obese women. Rev Bras Epidemiol 2015; 18:930–942.

Wen S, Niu Y, Lee SO, et al. Targeting fatty acid synthase with ASC-J9 suppresses proliferation and invasion of prostate cancer cells. Mol Carcinog 2016 Feb 19. doi: 10.1002/mc.22468

Harriman G, Greenwood J, Bhat S, et al. Acetyl-CoA carboxylase inhibition by ND-630 reduces hepatic steatosis, improves insulin sensitivity, and modulates dyslipidemia in rats. Proc Natl Acad Sci USA 2016; 113:E1796–1805.

Zhang JS, Lei JP, Wei GQ, Chen H, Ma CY, Jiang HZ . Natural fatty acid synthase inhibitors as potent therapeutic agents for cancers: a review. Pharm Biol 2016; 54:1919–1925.

Kuhajda FP, Pizer ES, Li JN, Mani NS, Frehywot GL, Townsend CA . Synthesis and antitumor activity of an inhibitor of fatty acid synthase. Proc Natl Acad Sci USA 2000; 97:3450–3454.

Hardwicke MA, Rendina AR, Williams SP, et al. A human fatty acid synthase inhibitor binds β-ketoacyl reductase in the keto-substrate site. Nat Chem Biol 2014; 10:774–779.

Otwinowski Z, Minor W . Processing of X-ray diffraction data collected in oscillation mode. Methods Enzymol 1997; 276:307–326.

Adams PD, Afonine PV, Bunkóczi G, et al. PHENIX: a comprehensive Python-based system for macromolecular structure solution. Acta Cryst D 2010; 66:213–221.

Emsley P, Cowtan K . Coot: model-building tools for molecular graphics. Acta Cryst D 2004; 60:2126–2132.

Jones G, Willett P, Glen RC, Leach AR, Taylor R . Development and validation of a genetic algorithm for flexible docking. J Mol Biol 1997; 267:727–748.

Konarev PV, Petoukhov MV, Volkov VV, Svergun DI . ATSAS 2.1, a program package for small-angle scattering data analysis. J Appl Crystallogr 2006; 9:277–286.

Konarev PV, Volkov VV, Sokolova AV, Koch MHJ, Svergun DI . PRIMUS: a Windows PC-based system for small-angle scattering data analysis. J Appl Crystallogr 2003; 36:1277–1282.

Svergun DI, Barberato C, Koch MHJ . CRYSOL - a program to evaluate X-ray solution scattering of biological macromolecules from atomic coordinates. J Appl Crystallogr 1995; 28:768–773.

Svergun DI . Determination of the regularization parameter in indirect-transform methods using perceptual criteria. J Appl Crystallogr 1992; 25:495–503.

Svergun DI . Restoring low resolution structure of biological macromolecules from solution scattering using simulated annealing. Biophys J 1999; 76:2879–2886.

Svergun DI, Petoukhov MV, Koch MHJ . Determination of domain structure of proteins from X-ray solution scattering. Biophys J 2001; 80:2946–2953.

Volkov VV, Svergun DI . Uniqueness of ab initio shape determination in small-angle scattering. J Appl Crystallogr 2003; 36:860–864.

Jerabek-Willemsen M, Wienken CJ, Braun D, Baaske P, Duhr S . Molecular interaction studies using microscale thermophoresis. Assay Drug Dev Technol 2011; 9:342–353.