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Bảo quản đông lạnh màng ối người cho tái tạo bề mặt mắt: so sánh giữa các giao thức
Tóm tắt
Nhằm so sánh tác động của các điều kiện bảo quản mới hơn đối với các tính chất kết dính và cấu trúc với những gì đạt được bằng một giao thức đã được thiết lập và tiêu chuẩn hóa (dimethyl sulfoxide ở −180 °C). Để đơn giản hóa và nâng cao tính an toàn của quy trình, chúng tôi đã thử nghiệm môi trường đông lạnh dựa trên dextran và điều kiện khô (không có môi trường) ở nhiệt độ −80 °C. Năm mẫu màng ối người được thu thập từ ba người hiến tặng khác nhau. Đối với mỗi người hiến, năm điều kiện bảo quản được thử nghiệm: dimethyl sulfoxide ở −180 °C, dimethyl sulfoxide ở −80 °C, môi trường dựa trên dextran ở −180 °C, môi trường dựa trên dextran ở −80 °C và đông lạnh khô ở −80 °C (không có môi trường). Vào cuối thời gian bảo quản bốn tháng, các tính chất kết dính và cấu trúc đã được phân tích. Không có giao thức bảo quản mới nào cho thấy sự khác biệt về tính chất kết dính và cấu trúc của các mô. Lớp stroma luôn giữ được tính kết dính, trong khi cả cấu trúc và màng đáy không bị thay đổi bởi bất kỳ giao thức bảo quản nào. Việc chuyển từ bảo quản đông lạnh bằng nitơ lỏng sang nhiệt độ −80 °C sẽ giảm thao tác, đơn giản hóa quy trình cũng như làm cho nó rẻ hơn. Việc sử dụng môi trường đông lạnh dựa trên dextran hoặc không sử dụng môi trường nào (điều kiện khô) sẽ tránh được độc tính tiềm ẩn của các môi trường đông lạnh dựa trên dimethyl sulfoxide.
Từ khóa
#bảo quản đông lạnh #màng ối người #tái tạo bề mặt mắt #dimethyl sulfoxide #dextran #tính chất kết dính #cấu trúc môTài liệu tham khảo
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