Cryopreservation enhances embryogenic capacity of Gentiana cruciata (L.) suspension culture and maintains (epi)genetic uniformity of regenerants

Anna Mikuła1, Karolina Tomiczak1, Jan J. Rybczyński1
1Botanical Garden–Center for Biological Diversity Conservation, Polish Academy of Sciences, ul. Prawdziwka 2, 02-973, Warsaw, Poland

Tóm tắt

Từ khóa


Tài liệu tham khảo

Aguilar ME, Engelmann F, Michaux-Ferrière N (1993) Cryopreservation of cell suspensions of Citrus deliciosa Tan. and histological study. Cryo Letters 14:217–228

Ahuja S, Mandal BB, Dixit S, Srivastava PS (2002) Molecular, phenotypic and biosynthetic stability in Dioscorea floribunda plants derived from cryopreserved shoot tips. Plant Sci 163:971–977

Bednarek P, Orłowska R, Koebner RMD, Zimny J (2007) Quantification of the tissue-culture induced variation in barley (Hordeum vulgare L.). BMC Plant Biol 7:10. doi: 10.1186/1471-2229-7-10

Channuntapipat C, Sedgley M, Collins G (2003) Changes in methylation and structure of DNA from almond tissues during in vitro culture and cryopreservation. J Am Soc Hort Sci 128:890–897

DeVerno LL, Park YS, Bonga JM, Barrett JD (1999) Somaclonal variation in cryopreserved embryogenic clones of white spruce [Picea glauca (Moench) Voss.]. Plant Cell Rep 18:948–953

Dixit S, Mandal BB, Ahuja S, Srivastava PS (2003) Genetic stability assessment of plants regenerated from cryopreserved embryogenic tissues of Dioscorea bulbifera L. using RAPD, biochemical and morphological analysis. Cryo Letters 24:77–84

Engelmann F (2004) Plant cryopreservation: progress and prospects. In Vitro Cell Dev Biol Plant 40:427–433

Engelmann F, Lartaud M, Chabrillange N, Carron MP, Etienne H (1997) Cryopreservation of embryogenic calluses of two commercial clones of Hevea brasiliensis. Cryo Letters 18:107–116

Fernandes P, Rodriguez E, Pinto G, Roldán-Ruiz I, De Loose M, Santos C (2008) Cryopreservation of Quercus suber somatic embryos by encapsulation-dehydration and evaluation of genetic stability. Tree Physiol 28:1841–1850

Fiuk A, Rybczyński JJ (2007) The effect of several factors on somatic embryogenesis and plant regeneration in protoplast cultures of Gentiana kurroo (Royle). Plant Cell Tiss Org Cult 91:263–271

Fiuk A, Rybczyński JJ (2008) Factor influencing efficiency of somatic embryogenesis of Gentiana kurroo (Royle) cell suspension. Plant Biotechnol Rep 2:33–39

Häggman HM, Ryynänen L, Aronen T, Krajnakowa J (1998) Cryopreservation of embryogenic cultures of Scots pine. Plant Cell Tiss Org Cult 54:45–53

Hao YJ, Liu QL, Deng XX (2001) Effect of cryopreservation on apple genetic resources at morphological, chromosomal and molecular levels. Cryobiology 43:46–53

Hao YJ, You CX, Deng XX (2002a) Analysis of ploidy and the patterns of amplified fragment length polymorphism and methylation sensitive amplified polymorphism in strawberry plants recovered from cryopreservation. Cryo Letters 23:37–46

Hao YJ, You CX, Deng XX (2002b) Effects of cryopreservation on developmental competency, cytological and molecular stability of citrus callus. Cryo Letters 23:27–35

Harding K (2004) Genetic integrity of cyopreseved plant cell: review. Cryo Letters 25:2–22

Johnston JW, Benson EE, Harding K (2009) Cryopreservation induces temporal DNA methylation epigenetic changes and differential transcriptional activity in Ribes germplasm. Plant Physiol Biochem 47:123–131

Kaity A, Ashmore SE, Drew RA, Dulloo ME (2008) Assessment of genetic and epigenetic changes following cryopreservation in papaya. Plant Cell Rep 27:1529–1539

Kaity A, Ashmore SE, Drew RA (2009) Field performance evaluation and genetic integrity assessment of cryopreserved papaya clones. Plant Cell Rep 28:1421–1430

Kamada H, Ishikawa K, Saga H, Harada H (1993) Induction of somatic embryogenesis in carrot by osmotic stress. Plant Cell Tiss Org Cult 10:38–44

Kikuchi A, Sanuki N, Higashi K, Koshiba T, Kamada H (2006) Abscisic acid and stress treatment are essential for the acquisition of embryogenic competence by carrot somatic cells. Planta 223:637–645

Lardet L, Martin F, Dessailly F, Carron MP, Montoro P (2007) Effect of exogenous calcium on post-thaw growth recovery and subsequent plant regeneration of cryopreserved embryogenic calli of Hevea brasiliensis (Mull. Arg.). Plant Cell Rep 26:559–569

Martín C, González-Benito ME (2005) Survival and genetic stability of Dendranthema grandiflora Tzvelev shoot apices after cryopreservation by vitrification and encapsulation–dehydration. Cryobiology 51:281–289

Martín C, González-Benito ME (2009) DNA sequences of variable bands found after cryopreservation of chrysanthemum apices. In: Programme and abstracts, 1st international symposium “Cryopreservation in horticultural species”, vol 28. Leuven, Belgia, 5–8 Apr 2009

Mikuła A (2006) Comparison of three techniques for cryopreservation and re-establishment of long-term Gentiana tibetica suspension culture. Cryo Letters 27:268–282

Mikuła A, Rybczyński JJ (2001) Somatic embryogenesis of Gentiana genus I. The effect of the preculture treatment and primary explant origin on somatic embryogenesis of Gentiana cruciata (L.), G. pannonica (Scop.) and G. tibetica (King). Acta Physiol Plant 23:15–25

Mikuła A, Fiuk A, Rybczyński JJ (2005a) Induction, maintenance and preservation of embryogenic competence of Gentiana cruciata L. cultures. Acta Biol Cracov Bot 47:227–236

Mikuła A, Rybczyński JJ, Skierski J, Latkowska MJ, Fiuk A (2005b) Somatic embryogenesis of Gentiana genus IV: Characterisation of Gentiana cruciata and Gentiana tibetica embryogenic cell suspensions. In: Hvolsef-Eide AK, Preil W (eds) Liquid culture systems for in vitro plant propagation. Springer, The Netherlands, pp 345–358

Mikuła A, Tykarska T, Kuraś M (2005c) Ultrastructure of Gentiana tibetica proembryogenic cells before and after cooling treatments. Cryo Letters 26:367–378

Mikuła A, Niedzielski M, Rybczyński JJ (2006) The use of TTC reduction assay for assessment of Gentiana spp. cell suspension viability after cryopreservation. Acta Physiol Plant 28:315–324

Mikuła A, Olas M, Sliwińska E, Rybczyński JJ (2008) Cryopreservation by encapsulation of Gentiana spp. cell suspensions maintains regrowth, embryogenic competence and DNA content. Cryo Letters 29:409–418

Moukadiri O, Lopes CT, Cornejo MJ (1999) Physiological and genomic variations in rice cells recovered from direct immersion and storage in liquid nitrogen. Physiol Plant 105:442–449

Peredo EL, Arroyo-García R, Reed BM, Revilla MÁ (2008) Genetic and epigenetic stability of cryopreserved and cold-stored hops (Humulus lupulus L.). Cryobiology 57:234–241

Peredo EL, Cires E, Arroyo-García R, Revilla MÁ (2009) Genetic and epigenetic stability of Humulus lupulus after in vitro culture. In: Proceedings of the scientific commission. International Hop Growers’ Convention I.H.G.C. León, Spain, 21–25 June 2009, pp 64–67. http://www.lfl.bayern.de/ipz/hopfen/10585/sc09_procceedings.pdf . Accessed 21 September 2010

Rybczyński JJ, Mikuła A, Tomiczak K, Wójcik A, Fiuk A (2008) Biotechnology of gentians: the induction of embryogenic potential, its cryopreservation and utilization in somatic cell genetic manipulation. Physiol Plant 133:P07–P055

Sales E, Nebauer SG, Arrillaga I, Segura J (2001) Cryopreservation of Digitalis obscura selected genotypes by encapsulation–dehydration. Planta Med 67:833–838

Sánchez C, Martínez MT, Vidal N, San-José MC, Valladares S, Vieitez AM (2008) Preservation of Quercus robur germplasm by cryostorage of embryogenic cultures derived from mature trees and RAPD analysis of genetic stability. Cryo Letters 29:493–504

Scocchi A, Faloci M, Medina R, Olmos S, Mroginski L (2004) Plant recovery of cryopreserved apical meristem-tips of Melia azedarach L. using encapsulation/dehydration and assessment of their genetic stability. Euphytica 135:29–38

Tanaka M, Kikuchi A, Kamada H (2009) Isolation of putative embryo-specific genes using stress induction of carrot somatic embryos. Breed Sci 59:37–46

Urbanová M, Košuth J, Čellárová E (2006) Genetic and biochemical analysis of Hypericum perforatum L. plants regenerated after cryopreservation. Plant Cell Rep 25:140–147

Walters C, Wheeler L, Stanwood PC (2004) Longevity of cryogenically stored seeds. Cryobiology 48:229–244

Wang Z, He Y (2009) Effect of cryopreservation on the development and DNA methylation patterns of Arabidopsis thaliana. Life Sci J 6:55–60

Wang ZY, Legris G, Nagel J, Potrykus I, Spangenberg G (1994) Cryopreservation of embryogenic cell suspensions in Festuca and Lolium species. Plant Sci 103:93–106

Wang Q, Gafny R, Sahar N, Sela I, Mawassi M, Tanne E, Perl A (2002) Cryopreservation of grapevine (Vitis vinifera L.) embryogenic cell suspensions by encapsulation-dehydration and subsequent plant regeneration. Plant Sci 162:551–558

Winkelmann T, Mußmann V, Serek M (2004) Cryopreservation of embryogenic suspension cultures of Cyclamen persicum Mill. Plant Cell Rep 23:1–8

Zaghmout OMF, Torello WA (1992) Restoration of regeneration potential of long-term cultures of red fescue (Festuca rubra L.) by elevated sucrose levels. Plant Cell Rep 11:142–145