Chiết xuất thô của baculovirus tái tổ hợp biểu hiện các hạt giống virus gây bệnh dịch truyền do virus hemorrhagic ở thỏ 2 từ cả tế bào côn trùng và thỏ bảo vệ thỏ chống lại bệnh dịch truyền do RHDV2

Archives of Virology - Tập 164 - Trang 137-148 - 2018
Claudia Müller1, Reiner Ulrich2, Kati Franzke3, Marcus Müller4, Bernd Köllner5
1Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institute, Greifswald-Insel Riems, Germany
2Department of Experimental Animal Facilities and Biorisk Management, Friedrich-Loeffler-Institute, Greifswald-Insel Riems, Germany
3Institute of Infectology, Friedrich-Loeffler-Institute, Greifswald-Insel Riems, Germany
4IDT Biologika Riems, Greifswald-Insel Riems, Germany
5Institute of Immunology, Friedrich-Loeffler-Institute, Greifswald-Insel Riems, Germany

Tóm tắt

Vắc xin chống lại các tác nhân gây bệnh virus thường được cấu thành từ các protein tái tổ hợp được biểu hiện trong các hệ thống khác nhau. Các protein này được biểu hiện bởi baculovirus tái tổ hợp đã được chứng minh là hiệu quả cho việc tiêm phòng. Đặc biệt, sau khi tối ưu hóa việc sử dụng mã codon, một lượng lớn protein virus tái tổ hợp có thể được thu hoạch, có khả năng tự lắp ráp thành các hạt giống virus tương tự (VLPs). Trong nghiên cứu này, chúng tôi đã so sánh hai loại mã codon khác nhau của RHDV2-VP1 để cải thiện sự biểu hiện của VP1 tái tổ hợp của RHDV2 thông qua baculovirus tái tổ hợp sau khi nhiễm tế bào SF9 của côn trùng hoặc chuyển giao vào tế bào RK13 của động vật có vú nhằm thu được năng suất protein cao. Ngoài ra, ảnh hưởng của việc tự lắp ráp RHDV2-VP1 thành VLPs cũng được nghiên cứu. Cuối cùng, khả năng gây miễn dịch của các vắc xin tái tổ hợp này chống lại RHDV2 để tạo ra phản ứng miễn dịch bảo vệ ở thỏ chống lại RHDV2 cần được đặc trưng. Không có ảnh hưởng của các loại mã codon khác nhau đối với sự biểu hiện gen RHDV2-VP1 trong các dòng tế bào tương ứng. Tuy nhiên, trong dòng tế bào côn trùng SF9, tỷ lệ VP1 tái tổ hợp được đo là cao hơn so với chuyển giao vào tế bào động vật có vú RK13. Việc tự lắp ráp RHDV2-VP1 thành VLPs đã được quan sát trong cả hai hệ tế bào thông qua kính hiển vi điện tử. Cuối cùng, cả VLPs RHDV-VP1 thu được từ tế bào động vật có vú và côn trùng đều có khả năng thúc đẩy phản ứng miễn dịch thể dịch bảo vệ ở thỏ chống lại RHDV2.

Từ khóa

#baculovirus tái tổ hợp #RHDV2-VP1 #VLPs #miễn dịch bảo vệ #dược phẩm thú y

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