Cross-reactivity of monoclonal antibodies againstClostridium perfringens Θ toxin with streptolysin O
Current Microbiology - 1984
Tóm tắt
Six monoclonal antibodies to Θ toxin ofClostridium perfringens were characterized. Four antibodies, 1C3, 2D4, 1B9, and 3F11, were nonneutralizing for Θ toxin and were non-cross-reacting with streptolysin O (SLO). The other two antibodies, 3H10 and 2C5, were cross-binding and cross-neutralizing with SLO. Neutralizing activity of 3H10 was higher than that of 2C5 on the basis of the binding activity with Θ toxin and SLO. Both antibodies could inhibit hemolysis even after binding of the toxins to sheep red blood cells and inhibited cardiotoxicity of the toxins in cultured heart cells.
Từ khóa
Tài liệu tham khảo
Alouf, J. E. 1976. Cell membranes and cytolytic bacterial toxins, pp. 221–270. In: Cuatrecasas, P. (ed.), The specificity and action of animal, bacterial and plant toxins. Receptors and recognition, Series B, vol. 1. London: Chapman and Hall.
Bernheimer, A. W. 1974. Interactions between membranes and cytolytic bacterial toxins. Biochimica et Biophysica Acta344:27–50.
Bernheimer, A. W. 1976. Sulfhydryl activated toxins. pp. 85–97. In: Bernheimer, A. W. (ed.), Mechanisms in bacterial toxinology. New York: John Wiley and Sons.
Cowell, J. L., Bernheimer, A. W. 1977. Antigenic relationships among thiol-activated cytolysins. Infection and Immunity16:397–399.
Ellman, G. L. 1959. Tissue sulfhydryl groups. Archives of Biochemistry and Biophysics82:70–77.
Ey, P. L., Prowse, S. J., Jenkin, C. R. 1978. Isolation of pure IgG1, IgG2a, and IgG2b immunoglobulins from mouse serum using protein A-Sepharose. Immunochemistry15:429–436.
Goding, J. W. 1980. Antibody production by hybridomas. Journal of Immunological Methods39:285–308.
Goshima, K. 1969. Synchronized beating of and electrotonic transmission between myocardial cells mediated by heterotypic strain cells in monolayer culture. Experimental Cell Research58:420–426.
Honda, T., Goshima, K., Takeda, Y., Sugino, Y., Miwatani, T. 1976. Demonstration of the cardiotoxicity of the thermostable direct hemolysin (lethal toxin) produced byVibrio parahaemolyticus. Infection and Immunity13:163–171.
Mansa, B., Kjems, E. 1970. Further studies on M-components with anti-streptolysin O activity. The inactivating effect on different oxygen-labile haemolysins. Acta Pathologica et Microbiologica, Scandinavica, SectionB 78:467–472.
Oberley, T. D., Duncan, J. L. 1971. Characteristics of streptolysin O action. Infection and Immunity4:683–687.
Oi, V. T., Herzenberg, L. A. 1980. Immunoglobulin producing hybrid cell lines. pp. 351–371. In: Mishell, B. B., Schiigi, S. W. (eds.), Selected methods in cellular immunology. San Francisco: W. H. Freeman.
Prigent, D., Alouf, J. E., Raynaud, M. 1974. Étude de la fixation de la streptolysine O radioiodée sur les érythrocytes. Comptes rendus des séances de l'académie des sciences, Série D278:651–653.
Sato, H., Sato, Y., Ito, A. 1983. Affinity of pertussis toxin produced byBordetella pertussis for human haptoglobin: application to the in vitro assay of the toxin. Journal of Microbiological Methods1:99–109.
Sato, H., Yamakawa, Y., Ito, A., Murata, R. 1979. Toxin-neutralizing effect of antibody against subtilisin-digested tetanus toxin. Infection and Immunity24:958–961.
Sato, Y., Cowell, J. L., Sato, H., Burstyn, D. G., Manclark, C. R. 1983. Separation and purification of the hemagglutinins fromBordetella pertussis. Infection and Immunity41:313–320.
Sekine, T., Ando, K. 1972. Fluorescent thiol reagents. V. Microfluometry of thiol compounds with a fluorescent-labeled maleimide. Analytical Biochemistry48:557–568.
Shany, S., Grushoff, P. S., Bernheimer, A. W. 1973. Physical separation of streptococcal nicotinamide adenine dinucleotide glycohydrolase from streptolysin O. Infection and Immunity7:731–734.
Smyth, C. J., Duncan, J. L. 1978. Thiol-activated (oxygen-labile) cytolysins, pp. 129–183. In: Jeljaszewicz, J., Wadström, T. (eds.), Bacterial toxins and cell membranes. London: Academic Press.
Voller, A., Bidwell, D., Bartlett, A. 1980. Enzyme-linked immunosorbent assay. pp. 359–371. In: Rose, N.R., Friedman, H. (eds.), Manual of clinical immunology. Washington, DC: American Society for Microbiology.
Yamakawa, Y., Ito, A., Sato, H. 1977. Theta-toxin ofClostridium perfringens. I. Purification and some properties. Biochimica et Biophysica Acta494:301–313.