CpG motifs present in bacteria DNA rapidly induce lymphocytes to secrete interleukin 6, interleukin 12, and interferon gamma.

Proceedings of the National Academy of Sciences of the United States of America - Tập 93 Số 7 - Trang 2879-2883 - 1996
Dennis M. Klinman1, Ae‐Kyung Yi2,3, Serge L. Beaucage2,3, Jacqueline Conover4, Arthur Μ. Krieg2,3
1Section of Retroviral Immunolgy, Division of Viral Products, Food and Drug Administration, Bethesda, MD 20892-4555, USA.
2University of Colorado, Boulder, CO
3tDepartment of Internal Medicine, Division of Rheumatology, University of Iowa College of Medicine and Veterans Affairs Medical Center, Iowa City, IA 52242
4Section of Retroviral Immunology, Division of Viral Products, and tDivision of Hematologic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892-4555;

Tóm tắt

Bacterial infection stimulates the host to mount a rapid inflammatory response. A 6-base DNA motif consisting of an unmethylated CpG dinucleotide flanked by two 5' purines and two 3' pyrimidines was shown to contribute to this response by inducing polygonal B-cell activation. This stimulatory motif is 20 times more common in the DNA of bacteria than higher vertebrates. The current work shows that the same motif induces the rapid and coordinated secretion of interleukin (IL) 6, IL-12, and interferon gamma (but not IL-2, IL-3, IL-4, IL-5, or IL-10) in vivo and in vitro. Stimulatory CpG DNA motifs induced B, T, and natural killer cells to secrete cytokine more effectively than did lipopolysaccharide. Thus, immune recognition of bacterial DNA may contribute to the cytokine, as well as the antibody production characteristic of an innate inflammatory response.

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Proceedings of the National Academy of Sciences of the United States of America

Tập 93 Số 7

2879-2883

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