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Cố định không đổi của invertase trên các hạt nano oxit sắt siêu từ tính được biến đổi bằng PAMAM-dendrimer
Tóm tắt
Nghiên cứu này tổng hợp chất PAMAM dendrimer trên bề mặt các hạt nano superparamagnetite để tăng cường sự cố định của invertase. Lượng enzyme được cố định trên bề mặt hạt nano magnetite phân nhánh siêu bão hòa cao gấp 2.5 lần (tức là 250%) so với hạt nano magnetite chỉ được biến đổi bằng aminosilane. Tốc độ phản ứng tối đa (V
max) và hằng số Michaelis–Menten (K
m) đã được xác định cho các enzyme tự do và enzyme đã được cố định. Nhiều đặc điểm của invertase được cố định như; hoạt động trong điều kiện nhiệt độ, độ ổn định nhiệt, độ ổn định trong hoạt động và độ ổn định khi bảo quản đã được đánh giá và kết quả cho thấy độ ổn định của enzyme được cải thiện khi cố định.
Từ khóa
#PAMAM #invertase #cố định enzyme #hạt nano #oxit sắt #superparamagnetiteTài liệu tham khảo
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