Cost Effective Production of Poly-β-Hydroxybutyrate by Bacillus subtilis NG05 Using Sugar Industry Waste Water

Journal of Polymers and the Environment - Tập 21 - Trang 441-449 - 2012
Gulab Singh1, Anish Kumari1, Arpana Mittal1, Varsha Goel1, Anita Yadav2, Neeraj Kumar Aggarwal1
1Department of Microbiology, Kurukshetra University, Kurukshetra, India
2Department of Biotechnology, Kurukshetra University, Kurukshetra, India

Tóm tắt

The present study describes the treatment of sugar industry waste water and its use as a potential low cost substrate for production of bioplastic by Bacillus subtilis NG05. The B. subtilis NG05 grow at the rate of 0.14 g h−1 L−1 of production media used and accumulate the 50.1 % of poly β-hydroxybutyrate (PHB). The phase contrast microscopy revealed the presence of PHB granules in B. subtilis NG05 which was further confirmed by Fourier transform infrared spectroscopy and 1H-nuclear magnetic resonance. The polymer was further analysed by differential scanning calorimetry. PHB production yield was achieved up to 4.991 g L−1 with Sugar industry waste water as sole nutrient source. Thus the process provided dual benefits of conversion of a waste material into value added product, PHB and waste management.

Tài liệu tham khảo

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