Correlation of human papillomavirus 16 and 18 with cervical neoplasia in histological typing and clinical stage in Taiwan: An in‐situ polymerase chain reaction approach

Journal of Surgical Oncology - Tập 78 Số 2 - Trang 101-109 - 2001
Jean‐Shiunn Shyu1, Cheng‐Jueng Chen2, Chia‐Chang Chiu3, Su‐Chin Huang4, Horng‐Jyh Harn5,4
1Department of Pathology, Armed Force Taoyuan Hospital, Taoyuan, Taiwan, ROC
2Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan, ROC
3Department of Surgery, Armed Force Taoyuan Hospital, Taoyuan, Taiwan, ROC
4Department of Pathology, Tri-Service General Hospital, Taipei, Taiwan, ROC
5Department of Emergency Medicine, Tri-Service General Hospital, Taipei, Taiwan, ROC

Tóm tắt

AbstractBackground and Objectives:In situ polymerase chain reaction (ISPCR) promises to considerably enhance our ability to detect a few copies of target nucleic acid sequences in fixed tissues and cells. The aim of this study was to investigate cervical carcinoma to determine the human papillomavirus (HPV) types on paraffin‐embedded tissue sections by ISPCR and standard in situ hybridization. The results will correlate the morphological characteristics of lesions with viral typing results..Methods:This study examined prevalence of HPV 16 and 18 DNA in biopsies from 85 cervical cancer patients by ISPCR, employing HPV 16, 18 consensus primers. There are 45 patients with squamous cell carcinomas, 13 with adenocarcinoma, 2 with adenosquamous carcinomas, 3 with small cell carcinomas, and 22 carcinoma in situ. The relation between the types of HPV detected, tumor type, and clinical stage were analyzed.Results:Fifty‐two of 85 biopsies were HPV 16‐ or 18‐positive, HPV 16 being the most prevalent type. Squamous cell carcinoma had a high prevalence of HPV 16 and adenocarcinoma had a high prevalence of HPV 18. HPV 18 was the predominant type among high clinical stage (III–IV) cases while HPV 16 and mixed HPV 16 with HPV18 were significantly correlated with low clinical stage (0–I–II).Conclusion:Our results indicate that certain malignant cervical tumor phenotypes and stages correlate with specific HPV type, and that ISPCR is a sensitive and fast method to detect HPV in these patients. J. Surg. Oncol. 2001;78:101–109. © 2001 Wiley‐Liss, Inc.

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Thông tin xuất bản

Journal of Surgical Oncology

Tập 78 Số 2

101-109

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