Coronin 3 and its role in murine brain morphogenesis

European Journal of Neuroscience - Tập 21 Số 5 - Trang 1155-1168 - 2005
Andreas Hasse1, André Rosentreter1, Ziqiang Spoerl1, Maria Stumpf1, Angelika A. Noegel1, Christoph S. Clemen1
1Center for Biochemistry and Center of Molecular Medicine Cologne, Medical Faculty, University of Cologne, Joseph-Stelzmann-Straße 52, D-50931 Koeln, Germany

Tóm tắt

Abstract

Coronins belong to the fundamental WD40‐repeat proteins. They are mainly found at the submembraneous area, they bind F‐actin in vitro, and most of the seven mammalian coronins have unclear roles. Coronin 3 is abundantly expressed in the adult CNS. All murine brain areas express coronin 3 during embryogenesis and the first postnatal stages. Expression in grey matter decreases postnatally, except for hippocampal pyramidal and dentate gyrus neurons, and cerebellar Purkinje cells, while levels in white matter increase in the course of myelination. Consistently, coronin 3 is abundant in differentiating neuro‐2a and PC‐12 cells and in primary oligodendrocytes. Treatment with PKC activator PMA reduced coronin 3 protein levels. To address its functions, neuro‐2a and PC‐12 cells were transfected with GFP‐tagged coronin 3 versions. Full‐length coronin 3 among other areas localized to outgrowing neurites, whereas truncated proteins efficiently suppressed neurite formation. Our results favour a role for coronin 3 in neuron morphogenesis and possibly migration.

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Thông tin xuất bản

European Journal of Neuroscience

Tập 21 Số 5

1155-1168

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