Copper stress and filamentous fungus<i>Humicola lutea</i>103 — ultrastructural changes and activities of key metabolic enzymes

Canadian Journal of Microbiology - Tập 58 Số 12 - Trang 1335-1343 - 2012
Ekaterina Ts. Krumova1, Stoyanka Stoitsova1, Tsvetelina Paunova‐Krasteva1, Svetlana B. Pashova1, Maria Angelova1
1The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 Academician G. Bonchev, 1113 Sofia, Bulgaria.

Tóm tắt

Humicola lutea 103 is a copper-tolerant fungal strain able to grow in the presence of 300 μg·mL–1Cu2+under submerged cultivation. To prevent the consequences of copper overload, microorganisms have evolved molecular mechanisms that regulate its uptake, intracellular traffic, storage, and efflux. In spite of this avoidance strategy, high heavy-metal concentrations caused distinct and widespread ultrastructural alterations in H. lutea. The mitochondria were the first and main target of the toxic action. The effect of copper on activities of the key enzymes (hexokinase, glucose-6-phosphate dehydrogenase, malate dehydrogenase, and isocitrate dehydrogenase) included in the 3 main metabolic pathways, glycolysis, pentose phosphate pathway, and tricarboxylic acid cycle, was investigated. High metal concentrations exhibited a dramatic negative effect on hexokinase, while the other 3 enzymes showed a significant and dose-dependent stimulation. On the basis of the present and previous results we concluded that the copper-induced oxidative stress plays an important role in the fungal tolerance to high Cu2+concentrations.

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Thông tin xuất bản

Canadian Journal of Microbiology

Tập 58 Số 12

1335-1343

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