Copper-Dependent Iron Assimilation Pathway in the Model Photosynthetic EukaryoteChlamydomonas reinhardtii

American Society for Microbiology - Tập 1 Số 5 - Trang 736-757 - 2002
Sharon La Fontaine1,2, Jeanette Quinn1, Stacie S. Nakamoto1, M. Dudley Page1, Vera Göhre1, Jeffrey L. Moseley1, Janette Kropat1, Sabeeha Merchant1,3
1Department of Chemistry and Biochemistry
2The Centre for Cellular and Molecular Biology, School of Biological and Chemical Sciences, Deakin University, Melbourne, Victoria, Australia
3Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California 90095-1569

Tóm tắt

ABSTRACTThe unicellular green algaChlamydomonas reinhardtiiis a valuable model for studying metal metabolism in a photosynthetic background. A search of theChlamydomonasexpressed sequence tag database led to the identification of several components that form a copper-dependent iron assimilation pathway related to the high-affinity iron uptake pathway defined originally forSaccharomyces cerevisiae. They include a multicopper ferroxidase (encoded byFox1), an iron permease (encoded byFtr1), a copper chaperone (encoded byAtx1), and a copper-transporting ATPase. A cDNA,Fer1, encoding ferritin for iron storage also was identified. Expression analysis demonstrated thatFox1andFtr1were coordinately induced by iron deficiency, as wereAtx1andFer1, although to lesser extents. In addition, Fox1 abundance was regulated at the posttranscriptional level by copper availability. Each component exhibited sequence relationship with its yeast, mammalian, or plant counterparts to various degrees; Atx1 ofC. reinhardtiiis also functionally related with respect to copper chaperone and antioxidant activities. Fox1 is most highly related to the mammalian homologues hephaestin and ceruloplasmin; its occurrence and pattern of expression inChlamydomonasindicate, for the first time, a role for copper in iron assimilation in a photosynthetic species. Nevertheless, growth ofC. reinhardtiiunder copper- and iron-limiting conditions showed that, unlike the situation in yeast and mammals, where copper deficiency results in a secondary iron deficiency, copper-deficientChlamydomonascells do not exhibit symptoms of iron deficiency. We propose the existence of a copper-independent iron assimilation pathway in this organism.

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American Society for Microbiology

Tập 1 Số 5

736-757

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