Cooperative binding of ATP and MgADP in the sulfonylurea receptor is modulated by glibenclamide

Proceedings of the National Academy of Sciences of the United States of America - Tập 96 Số 4 - Trang 1268-1272 - 1999
Kazumitsu Ueda1, Jun Komine1, Masaru Matsuo1, Susumu Seino1, Teruo Amachi1
1Laboratory of Biochemistry, Division of Applied Life Sciences, Kyoto University Graduate School of Agriculture, Kyoto 606-8502, Japan; and Department of Molecular Medicine, Chiba University Graduate School of Medicine, Chuo-ku, Chiba 260-8670, Japan

Tóm tắt

The ATP-sensitive potassium (K ATP ) channels in pancreatic β cells are critical in the regulation of glucose-induced insulin secretion. Although electrophysiological studies provide clues to the complex control of K ATP channels by ATP, MgADP, and pharmacological agents, the molecular mechanism of K ATP -channel regulation remains unclear. The K ATP channel is a heterooligomeric complex of SUR1 subunits of the ATP-binding-cassette superfamily with two nucleotide-binding folds (NBF1 and NBF2) and the pore-forming Kir6.2 subunits. Here, we report that MgATP and MgADP, but not the Mg salt of γ-thio-ATP, stabilize the binding of prebound 8-azido-[α- 32 P]ATP to SUR1. Mutation in the Walker A and B motifs of NBF2 of SUR1 abolished this stabilizing effect of MgADP. These results suggest that SUR1 binds 8-azido-ATP strongly at NBF1 and that MgADP, either by direct binding to NBF2 or by hydrolysis of bound MgATP at NBF2, stabilizes prebound 8-azido-ATP binding at NBF1. The sulfonylurea glibenclamide caused release of prebound 8-azido-[α- 32 P]ATP from SUR1 in the presence of MgADP or MgATP in a concentration-dependent manner. This direct biochemical evidence of cooperative interaction in nucleotide binding of the two NBFs of SUR1 suggests that glibenclamide both blocks this cooperative binding of ATP and MgADP and, in cooperation with the MgADP bound at NBF2, causes ATP to be released from NBF1.

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Proceedings of the National Academy of Sciences of the United States of America

Tập 96 Số 4

1268-1272

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