Controlled release of complexed DNA from polycaprolactone film: Comparison of lipoplex and polyplex release

Journal of Biomedical Materials Research - Part B Applied Biomaterials - Tập 89B Số 2 - Trang 439-447 - 2009
Yamini Ramgopal1, Debasish Mondal1, Subbu S. Venkatraman1, W.T. Godbey2, Gan Yik Yuen3
1Division of Materials Technology, School of Materials Science and Engineering, Nanyang Technological University, Singapore 637819.
2Department of Chemical and Biomolecular Engineering, Tulane University, New Orleans, Louisiana 70118
3Department of Natural Sciences and Science Education, National Institute of Education, Nanyang Technological University, Singapore 637616

Tóm tắt

AbstractThis report investigates the comparative in vitro controlled release and transfection efficiencies of pDNA‐lipofectamine complex (lipoplex) and pDNA‐poly(ethylene imine) complex (polyplex), from a biodegradable polycaprolactone (PCL) film. The effect of molecular weight of gelatin used as a porogen on in vitro release and transfection efficiency was also studied. A sustained release profile was obtained for naked pDNA and lipoplex from polymeric films for a month, while the release of polyplexes (PEI/DNA) is simply a burst at day 5, with little or no release thereafter. The release of polyplexes from PCL films is retarded due to interaction between the polyplexes and the polymer. A high burst release was seen for naked pDNA which was suppressed in the presence of gelatin. The extent of suppression of the burst effect by gelatin increased with its molecular weight. For complexed pDNA (lipoplex), the release was slow, but could be accelerated using gelatin; again the acceleration in release is dependant on the molecular weight of the gelatin used. The addition of gelatin as a porogen has no effect on the release of polyplexes from PCL films. The bioactivity of released plasmid DNA and complexes was studied by in vitro transfection using COS‐7 cells. Transfection was observed from released lipoplexes samples till day 9 from PCL film with lower MW gelatin and till day 18 in the case of PCL films with higher MW gelatin. The results also showed that the bioactivity of released lipoplexes was superior to that of the naked pDNA. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009

Từ khóa


Tài liệu tham khảo

10.1038/nm0397-357

10.1615/CritRevTherDrugCarrierSyst.v15.i2.20

10.1208/aapsj070109

10.1002/jbm.b.30971

10.1016/S0142-9612(00)00101-0

Kohn J, 1996, Biomaterials Science: An Introduction to Materials In Medicine, 64

10.1016/0142-9612(81)90060-0

10.1002/jbm.10120

10.1016/S0169-409X(97)00125-7

10.1016/j.jconrel.2005.09.023

10.1002/app.22289

10.1038/9853

10.1016/S0168-3659(99)00151-0

Howard KA, 2004, Formulation of a microparticle carrier for oral polyplexes ‐ based DNA vaccines, Biochim Biophys Acta, 1674, 149

Aral C, 2000, Studies of plasmid DNA‐loaded chitosan microspheres. I. Plasmid size, chitosan concentration and plasmid addition techniques, STP Pharm Sci, 10, 83

10.1016/S0168-3659(00)00361-8

10.1016/j.jconrel.2003.11.001

10.1016/S0939-6411(03)00187-5

10.1002/0470011149

10.1002/jps.10437

10.1002/jps.10533

Thông tin
Thông tin xuất bản

Journal of Biomedical Materials Research - Part B Applied Biomaterials

Tập 89B Số 2

439-447

Thông tin tác giả