Controlled Intracellular Processing of Fusion Proteins by TEV Protease
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Baneyx, 1999, Recombinant protein expression in Escherichia coli, Curr. Opin. Biotechnol., 10, 411, 10.1016/S0958-1669(99)00003-8
Nilsson, 1997, Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins, Protein Express. Purif., 11, 1, 10.1006/prep.1997.0767
Maina, 1988, A vector to express and purify foreign proteins in Escherichia coli by fusion to, and separation from, maltose binding protein, Gene, 74, 365, 10.1016/0378-1119(88)90170-9
Smith, 1988, Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase, Gene, 67, 31, 10.1016/0378-1119(88)90005-4
Martinez, 1995, Expression of recombinant human phenylalanine hydroxylase as fusion protein in Escherichia coli circumvents proteolytic degradation by host cell proteases: Isolation and characterization of the wild-type enzyme, Biochem. J., 306, 589, 10.1042/bj3060589
Jacquet, 1999, Expression of a recombinant Toxoplasma gondii ROP2 fragment as a fusion protein in bacteria circumvents insolubility and proteolytic degradation, Protein Express. Purif., 17, 392, 10.1006/prep.1999.1150
Butt, 1989, Ubiquitin fusion augments the yield of cloned gene products in Escherichia coli, Proc. Natl. Acad. Sci. USA, 86, 2540, 10.1073/pnas.86.8.2540
Pryor, 1997, High-level expression of soluble protein in Escherichia coli using a His6-tag and maltose-binding-protein double-affinity fusion system, Protein Express. Purif., 10, 309, 10.1006/prep.1997.0759
Kapust, 1999, Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused, Protein Sci., 8, 1668, 10.1110/ps.8.8.1668
Park, 1998, Maltose binding protein (MBP) fusion proteins with low or no affinity to amylose resins can be single-step purified using a novel anti-MBP monoclonal antibody, Mol. Cells, 8, 709
Mondigler, 1996, Site-specific proteolysis of the Escherichia coli SecA protein in vivo, J. Bacteriol., 178, 2986, 10.1128/jb.178.10.2986-2988.1996
Lutz, 1997, Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements, Nucleic Acids Res., 25, 1203, 10.1093/nar/25.6.1203
Miller, 1972
Dougherty, 1989, Molecular genetic analysis of a plant virus polyprotein cleavage site: A model, Virology, 171, 356, 10.1016/0042-6822(89)90603-X
Smith, 1991, Direct selection for sequences encoding proteases of known specificity, Proc. Natl. Acad. Sci. USA, 88, 5159, 10.1073/pnas.88.12.5159
Studier, 1990, Use of T7 RNA polymerase to direct expression of cloned genes, Methods Enzymol., 185, 60, 10.1016/0076-6879(90)85008-C
Gupta, 1999, Enhancing recombinant protein yields in Escherichia coli using the T7 system under the control of heat inducible lambda PL promoter, J. Biotechnol., 68, 125, 10.1016/S0168-1656(98)00193-X
Vasina, 1996, Recombinant protein expression at low temperatures under the transcriptional control of the major Escherichia coli cold shock promoter cspA, Appl. Environ. Microbiol., 62, 1444, 10.1128/AEM.62.4.1444-1447.1996
de Boer, 1983, The tac promoter: A functional hybrid derived from the trp and lac promoters, Proc. Natl. Acad. Sci. USA, 80, 21, 10.1073/pnas.80.1.21
Elvin, 1990, Modified bacteriophage lambda promoter vectors for overproduction of proteins in Escherichia coli, Gene, 87, 123, 10.1016/0378-1119(90)90503-J
Bhandari, 1997, An Escherichia coli host strain useful for efficient overproduction of cloned gene products with NaCl as the inducer, J. Bacteriol., 179, 4403, 10.1128/jb.179.13.4403-4406.1997
Perez-Perez, 1995, An arabinose-inducible expression vector, pAR3, compatible with ColE1-derived plasmids, Gene, 158, 141, 10.1016/0378-1119(95)00127-R
Newman, 1999, Broad-host-range expression vectors that carry the l-arabinose-inducible Escherichia coli araBAD promoter and the araC regulator, Gene, 227, 197, 10.1016/S0378-1119(98)00601-5
Guzman, 1995, Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter, J. Bacteriol., 177, 4121, 10.1128/jb.177.14.4121-4130.1995
Seigle, 1998, Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations, Proc. Natl. Acad. Sci. USA, 94, 8168, 10.1073/pnas.94.15.8168
Degenkolb, 1991, Structural requirements of tetracycline-Tet repressor interaction: Determination of equilibrium binding constants for tetracycline analogs with the Tet repressor, Antimicrob. Agents Chemother., 35, 1591, 10.1128/AAC.35.8.1591
Davison, 1984, Mechanism of control of DNA replication and incompatibility in ColE1-type plasmids–A review, Gene, 28, 1, 10.1016/0378-1119(84)90082-9
Prasher, 1992, Primary structure of the Aequorea victoria green-fluorescent protein, Gene, 111, 229, 10.1016/0378-1119(92)90691-H
Williamson, 1994, Solution structure of the active domain of tissue inhibitor of metalloproteinases-2: A new member of the OB fold protein family, Biochemistry, 33, 11745, 10.1021/bi00205a010
Serrano, 1993, A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4, Nature, 366, 704, 10.1038/366704a0
Vousden, 1993, Interactions of human papillomavirus transforming proteins with the products of tumor suppressor genes, FASEB J., 7, 872, 10.1096/fasebj.7.10.8393818
Hammarberg, 1989, Dual affinity fusion approach and its use to express recombinant insulin-like growth factor II, Proc. Natl. Acad. Sci. USA, 86, 4367, 10.1073/pnas.86.12.4367
Panagiotidis, 1995, PALEX, a dual-tag prokaryotic expression vector for the purification of full-length proteins, Gene, 164, 45, 10.1016/0378-1119(95)00417-5
Murby, 1991, Stabilization of recombinant proteins from proteolytic degradation in Escherichia coli using a dual affinity fusion strategy, Biotechnol. Appl. Biochem., 14, 336, 10.1111/j.1470-8744.1991.tb00186.x