Continuous production of somatomedin C with immobilized transformed yeast cells
Tóm tắt
Yeast cells producing the growth hormone somatomedin C (SMC) were constructed and applied in the immobilized form continuously for a period of over 10 days in a flow-through bioreactor. The construction of the MFα1-SMC fusion vector p336/1 is given as well as the results of the influence of various nutrients effecting hormone production. Immobilization of the transformed yeast cells is described and their application in a continuous bioreactor system. This study demonstrates the feasibility of a longterm and high-level hormone production by immobilized transformed yeast. The SMC productivities of free cells in batch and immobilized cells under continuous conditions were 0.2–0.3 and 0.5–0.6 mg per g wet cells and day, respectively.
Tài liệu tham khảo
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