Considerations regarding use of solvents in in vitro cell based assays

Michael Timm1, Lasse Saaby2, Lise Moesby1, Erik W. Hansen1
1Department of Pharmacology and Pharmacotherapy, Faculty of Pharmaceutical Sciences, University of Copenhagen, Universitetsparken 2, K-2100, Copenhagen, Denmark
2Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Universitetsparken 2, K-2100, Copenhagen, Denmark

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Desy O, Carignan D, Caruso M, de Campos-Lima PO (2008) Immunosuppressive effect of isopropanol: down-regulation of cytokine production results from the alteration of discrete transcriptional pathways in activated lymphocytes. J Immunol 181:2348–2355

Desy O, Carignan D, Caruso M, de Campos-Lima PO (2010) Methanol induces a discrete transcriptional dysregulation that leads to cytokine overproduction in activated lymphocytes. Toxicol Sci 117:303–313

Forman S, Kas J, Fini F, Steinberg M, Ruml T (1999) The effect of different solvents on the ATP/ADP content and growth properties of HeLa cells. J Biochem Mol Toxicol 13(11–15):3

Hollebeeck S, Raas T, Piront N, Schneider YJ, Toussaint O, Larondelle Y, During A (2011) Dimethyl sulfoxide (DMSO) attenuates the inflammatory response in the in vitro intestinal Caco-2 cell model. Toxicol Lett 206:268–275

Irie T, Uekama K (1997) Pharmaceutical applications of cyclodextrins.3. Toxicological issues and safety evaluation. J Pharm Sci 86:147–162

Kahler CP (2000) Evaluation of the use of the solvent dimethyl sulfoxide in chemiluminescent studies. Blood Cells Mol Dis 26:626–633

Moesby L, Timm M, Hansen EW (2008) Effect of moist 370 heat sterilisation on the pyrogenicity of cell wall components from Staphylococcus aureus. Eur J Pharm Sci 35:442–446

Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63

Nair MP, Kronfol ZA, Schwartz SA (1990) Effects of alcohol and nicotine on cytotoxic functions of human lymphocytes. Clin Immunol Immnopathol 54:395–409

Stella VJ, He Q (2008) Cyclodextrins. Toxicol Pathol 36:30–42

Strober W (2001) Trypan blue exclusion test of cell viability. Curr Protoc Immunol Appendix 3, A.3B.1–A.3B.2

Szabo G (1999) Consequences of alcohol consumption on host defence. Alcohol Alcohol 34:830–841

Timm M, Madsen AM, Hansen JV, Moesby L, Hansen EW (2009) Assessment of total inflammatory potential of bioaerosols using a granulocyte assay. Appl Environ Microbiol 75(24):7655–7662

Wakabayashi I, Negoro M (2002) Mechanism of inhibitory action of ethanol on inducible nitric oxide synthesis in macrophages. Naunyn-Schmiedeberg’s Arch Pharmacol 366:299–306

Xing L, Remick DG (2005) Mechanisms of dimethyl sulfoxide augmentation of IL-1 beta production. J Immunol 174:6195–6202

Zhijun L (2008) Preparation of botanical samples for biomedical research. Endocr Metab Immune Disord Drug Targets 8:112–121