Confirmation of “pre-plasmolysis mediated ex-osmosis hypothesis” to obtain shoot bud morphogenesis in Catharanthus roseus
Tóm tắt
The antineoplastic herb, Catharanthus roseus is a classified high-value low-volume medicinal herb which is in global attention of scientific research for modulation of its monoterpenoid indole alkaloids (MIA) pathway through genetic engineering. These secondary metabolites are generally stored in specific types of structures/compartments due to their cytotoxic nature and designated roles in plant defense response. However, their presence can hinder the genetic engineering process used to develop transgenic plants through de novo morphogenesis and regeneration of plants from cultured cells/tissues and hence, it always remained a critical impediment in transgenic research in C. roseus. The pre-plasmolysis treatment of leaf explants can help to tackle the recalcitrant nature of leaf explant and can support the direct regeneration response by ex-osmosis that minimizes the concentration of alkaloids. Therefore, this study was performed to chase the effect of osmotic conditions on recalcitrant leaves of C. roseus engaged in vitro plant regeneration and hypothesis of alkaloids ex-osmosis is confirmed by HPLC analysis.
Tài liệu tham khảo
citation_journal_title=Annu Rev Cell Biol; citation_title=Signal transduction in guard cells; citation_author=SM Assmann; citation_volume=9; citation_issue=1; citation_publication_date=1993; citation_pages=345-375; citation_doi=10.1146/annurev.cb.09.110193.002021; citation_id=CR1
citation_journal_title=Sci Agric (Piracicaba, Braz); citation_title=Anatomical and physiological modifications of micropropagated ‘Caipira’ banana plants under natural light; citation_author=FHS Costa, M Pasqual, JES Pereira, EM Castro; citation_volume=66; citation_publication_date=2009; citation_pages=323-330; citation_doi=10.1590/S0103-90162009000300007; citation_id=CR2
citation_journal_title=Phytochem Rev; citation_title=Catharanthus terpenoid indole alkaloids: biosynthesis and regulation; citation_author=M El-Sayed, R Verpoorte; citation_volume=6; citation_issue=2-3; citation_publication_date=2007; citation_pages=277-305; citation_doi=10.1007/s11101-006-9047-8; citation_id=CR3
citation_journal_title=Dev Biol; citation_title=The isolation, culture and regeneration of petunia leaf protoplast; citation_author=null Fearson; citation_volume=33; citation_issue=1; citation_publication_date=1973; citation_pages=130-137; citation_doi=10.1016/0012-1606(73)90169-3; citation_id=CR4
citation_journal_title=Plant Cell Physiol; citation_title=Osmotic stress induces inactivation of photosynthesis in guard cell protoplasts of Vicia leaves; citation_author=CH Goh, R Hedrich, U Schreiber; citation_volume=42; citation_issue=10; citation_publication_date=2001; citation_pages=1186-1191; citation_doi=10.1093/pcp/pce149; citation_id=CR5
citation_journal_title=Comb Proc Int Plant Prop Soc Proc; citation_title=Commercially-feasible micropropagation of mountain laurel, Kalmia atifolia by use of shoot-tip culture; citation_author=G Lloyd, BH McCown; citation_volume=30; citation_publication_date=1980; citation_pages=421-427; citation_id=CR6
citation_journal_title=Plant J; citation_title=ABA-induced ion efflux in stomatal guard cells: multiple actions of ABA inside and outside the cell; citation_author=EAC MacRobbie; citation_volume=7; citation_issue=4; citation_publication_date=1995; citation_pages=565-576; citation_doi=10.1046/j.1365-313X.1995.7040565.x; citation_id=CR7
citation_journal_title=Physiol Plant; citation_title=A revised medium for rapid growth and bioassays with tobacco tissue cultures; citation_author=T Murashige, F Skoog; citation_volume=15; citation_issue=3; citation_publication_date=1962; citation_pages=473-497; citation_doi=10.1111/j.1399-3054.1962.tb08052.x; citation_id=CR8
citation_journal_title=Biotechnol Lett; citation_title=Present status of Catharanthus roseus monoterpenoid indole alkaloids engineering in homo- and hetero-logous systems; citation_author=A Sharma, D Amin, A Sankaranarayanan, R Arora, AK Mathur; citation_volume=42; citation_issue=1; citation_publication_date=2020; citation_pages=11-23; citation_doi=10.1007/s10529-019-02757-4; citation_id=CR9
citation_journal_title=Protoplasma; citation_title=Genetic engineering approach using early Vinca alkaloid biosynthesis genes led to increased tryptamine and terpenoid indole alkaloids biosynthesis in differentiating cultures of Catharanthus roseus; citation_author=A Sharma, P Verma, A Mathur, AK Mathur; citation_volume=255; citation_publication_date=2017; citation_pages=425-435; citation_doi=10.1007/s00709-017-1151-7; citation_id=CR10
citation_journal_title=Protoplasma; citation_title=Overexpression of tryptophan decarboxylase and strictosidine synthase enhanced terpenoid indole alkaloids pathway activity and antineoplastic vinblastine biosynthesis in Catharanthus roseus; citation_author=A Sharma, P Verma, A Mathur, AK Mathur; citation_volume=255; citation_issue=5; citation_publication_date=2018; citation_pages=1281-1294; citation_doi=10.1007/s00709-018-1233-1; citation_id=CR11
citation_journal_title=Curr Med Chem; citation_title=The Catharanthus alkaloids: pharmacognosy and biotechnology; citation_author=JDI Heijden, W Snoeijer, D Hallard, R Verpoorte; citation_volume=11; citation_issue=5; citation_publication_date=2004; citation_pages=607-628; citation_doi=10.2174/0929867043455846; citation_id=CR12
citation_journal_title=Plant Cell Tissue Organ Cult; citation_title=Direct shoot bud organogenesis and plant regeneration from leaf explants in Catharanthus roseus; citation_author=P Verma, AK Mathur; citation_volume=106; citation_publication_date=2011; citation_pages=401-408; citation_doi=10.1007/s11240-011-9936-4; citation_id=CR13
citation_journal_title=Biotechnol Lett; citation_title=Agrobacterium tumefaciens-mediated transgenic plant production via direct shoot bud organogenesis from pre-plasmolyzed leaf explants of Catharanthus roseus; citation_author=P Verma, AK Mathur; citation_volume=33; citation_issue=5; citation_publication_date=2011; citation_pages=1053-1060; citation_doi=10.1007/s10529-010-0515-2; citation_id=CR14