Composition of volatiles from fermenting pollen dough and attractiveness to the small hive beetle Aethina tumida, a parasite of the honeybee Apis mellifera
Tóm tắt
The response of the small hive beetle (SHB), Aethina tumida Murray, to volatiles from a pollen-based diet conditioned by the feeding of 100 adult virgin female or male SHBs (4–8 weeks old) for 1, 3, 7 or 14 days is described and compared to that of the same diet inoculated with the yeast Kodamaea ohmeri (NRRL Y-30722), isolated from the beetle. In a wind tunnel, volatiles from pollen dough conditioned by beetles of either sex for 3 or 7 days lured significantly more beetles into traps than volatiles from unconditioned dough. In contrast, trap captures with volatiles from dough conditioned for 1 and 14 days were weakly attractive. In cage bioassays, when naïve, unfed, virgin, SHBs (3–4 days old) were given a choice between yeast-inoculated pollen dough and non-inoculated dough, the responses were similar to those obtained in the wind tunnel with dough conditioned by SHBs for 3 and 7 days. Chemical analysis revealed high levels of fermentation-related products in volatiles that attracted the beetle.
Tài liệu tham khảo
Bartelt R.J., Seaton K.L., Dowd P.F. (1993) Aggregation pheromone of Carpophilus antiquus (Coleoptera: Nitidulidae) and kairomonal use of C. lugubris pheromone by C. antiquus, J. Chem. Ecol. 10, 2203–2216.
Bartelt R.J., Zilkowski B.W. (1998) A versatile and quantitative volatile-delivery system for laboratory bioassays and examples of use with host-related attractants for Carpophilus humeralis, J. Chem. Ecol. 24, 535–558.
Charpentier R., Weibull J. (1994) Secondary sexual differences of pollen beetle Meligethes aeneus Fabr. (Coleoptera:Nitidulidae) pupae, J. Appl. Entomol. 118, 100–102.
Elzen P.J., Baxter J.R., Westervelt D., Randall C., Delaplane K.S., Cutts L., Wilson W.T. (1999) Field control and biology studies of a new pest species, Aethina tumida Murray (Coleoptera, Nitidulidae), attacking European honeybees in the western hemisphere, Apidologie 31, 361–366.
Ephrussi B., Beadle G.W. (1936) A technique of transplantation for Drosophila, Am. Nat. 70, 218–225.
Hood M. (2004) The small hive beetle, Aethina tumida: a review, Bee World 85, 51–59.
James D.G., Bartelt R.J., Moore CJ. (1996) Trap design effect on capture of Carpophilus spp. (Coleoptera: Nitidulidae) using synthetic aggregation pheromone and a coattractant, Ecol. Behav. 89, 648–653.
Lin H., Phelan L. (1991) Identification of food volatiles attractive to dusky sap beetle, Carpophilus lugubris (Coleoptera: Nitidulidae), J. Chem. Ecol. 17, 1273–1286.
Nout M.R., Bartelt RJ. (1998) Attraction of a flying nitidulid (Carpophilus humeralis) to volatiles produced by yeasts grown on sweet corn and a corn-based medium, J. Chem. Ecol. 24, 1217–1239.
Phelan L., Lin H. (1991) Chemical characterization of fruit and fungal volatiles attractive to dried-fruit beetle, Carpophilus hemiptera (Coleoptera: Nitidulidae), J. Chem. Ecol. 17, 1253–1272.
Smilanick J.M., Ehler L.E., Birch M.C. (1978) Attraction of Carpophilus spp. (Coleoptera: Nitidulidae) to volatile compounds present in figs, J. Chem. Ecol. 4, 701–707.
Suazo A., Torto B., Teal P.E.A., Tumlinson J.H. (2003) Response of the small hive beetle (Aethina tumida) to honey bee (Apis mellifera) and beehive-produced volatiles, Apidologie 34, 525–533.
Teal P.E.A, Torto B., Boucias D., Tumlinson J.H. (2006) In-hive trap and attractant composition for control of the small hive beetle, Aethina tumida, US Patent: 20060141904.
Torto B., Suazo A., Alborn H., Teal P.E.A., Tumlinson J.H. (2005) Response of the small hive beetle (Aethina tumida) to a blend of chemicals identified from honeybee (Apis mellifera) volatiles, Apidologie 36, 523–532.
U. S. Department of Commerce (2005) NIST/EPA/ NIH mass spectral library (NIST05), J. Wiley, New York, [online] http://www.nist.gov/srd/nistla.htm (accessed on 3 May 2007).
Zhu J., Park K., Baker T.C. (2003) Identification of odors from overripe mango that attract vinegar flies, Drosophila melanogaster, J. Chem. Ecol. 29, 899–909.
Zilkowski B.W., Bartelt R.J., Blumberg D., James D.G., Weaver D.K. (1999) Identification of host-related volatiles attractive to pineapple beetle Carpophilus humeralis, J. Chem. Ecol. 25, 229–252.