Complete genome sequence of the industrial l-lysine production strain [Brevibacterium] flavum CCM 251
Tóm tắt
[Brevibacterium] flavum CCM 251 is used to produce l-lysine in the Biotika biotech factory in Slovenská Ľupča, Slovakia. [B.] flavum CCM 251 is the exclusive host of the lytic corynephage BFK20, which has been extensively studied. We have sequenced the complete genome of [B.] flavum CCM 251 using a combination of the Illumina MiSeq platform and MinION technology. The genome comprised a circular chromosome of 3362281 bp with 54.13% G + C content. In this study, we present an analysis of this genome. We identified six prophage-related sequence regions; three of these regions were classified as incomplete and the three others as questionable. The prophages were classified as Caudovirales, family Siphoviridae. In silico analyses of the genome identified two complete aspartate biosynthetic pathways involved in l-lysine production, a direct variant and a succinylase variant. The complete genome sequence of [B.] flavum CCM 251 (GenBank, CP079240) was compared with the genomes of other Corynebacterial strains. We confirmed these related strains have high similarity: 98.12% homology to C. glutamicum ATCC 13032 (EMBL BA00036) and 99.98% homology to [B.] flavum ATCC 15168 and C. glutamicum BE.
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