Complete genome sequence of <i>Lactobacillus plantarum</i> WCFS1

Proceedings of the National Academy of Sciences of the United States of America - Tập 100 Số 4 - Trang 1990-1995 - 2003
Michiel Kleerebezem1, Jos Boekhorst1, Richard van Kranenburg1, Douwe Molenaar2,1, Oscar P. Kuipers1, Rob J. Leer1, Renato Tarchini1, Sander Peters1, H. Sandbrink1, Mark Fiers1, Willem J. Stiekema1, R.M. Klein Lankhorst1, Peter A. Bron1, Sally M. Hoffer1, M.N. Nierop Groot3,1, Robert Kerkhoven1, Maaike de Vries1, Björn M. Ursing1, Willem M. de Vos1, Roland J. Siezen4,1
1Wageningen Centre for Food Sciences, P.O. Box 557, 6700 AN Wageningen, The Netherlands; Greenomics, Plant Research International, P.O. Box 16, 6700 AA Wageningen, The Netherlands; and Center for Molecular and Biomolecular Informatics, University of Nijmegen, P.O. Box 9010, 6500GL Nijmegen, The Netherlands
2Systems Bioinformatics
3Art and Culture, History, Antiquity
4Wageningen Centre for Food Sciences, P.O. Box 557, 6700 AN Wageningen, The Netherlands

Tóm tắt

The 3,308,274-bp sequence of the chromosome of Lactobacillus plantarum strain WCFS1, a single colony isolate of strain NCIMB8826 that was originally isolated from human saliva, has been determined, and contains 3,052 predicted protein-encoding genes. Putative biological functions could be assigned to 2,120 (70%) of the predicted proteins. Consistent with the classification of L. plantarum as a facultative heterofermentative lactic acid bacterium, the genome encodes all enzymes required for the glycolysis and phosphoketolase pathways, all of which appear to belong to the class of potentially highly expressed genes in this organism, as was evident from the codon-adaptation index of individual genes. Moreover, L. plantarum encodes a large pyruvate-dissipating potential, leading to various end-products of fermentation. L. plantarum is a species that is encountered in many different environmental niches, and this flexible and adaptive behavior is reflected by the relatively large number of regulatory and transport functions, including 25 complete PTS sugar transport systems. Moreover, the chromosome encodes >200 extracellular proteins, many of which are predicted to be bound to the cell envelope. A large proportion of the genes encoding sugar transport and utilization, as well as genes encoding extracellular functions, appear to be clustered in a 600-kb region near the origin of replication. Many of these genes display deviation of nucleotide composition, consistent with a foreign origin. These findings suggest that these genes, which provide an important part of the interaction of L. plantarum with its environment, form a lifestyle adaptation region in the chromosome.

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Proceedings of the National Academy of Sciences of the United States of America

Tập 100 Số 4

1990-1995

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