Complete genome sequence of Stellaria aquatica virus B, a novel polerovirus that infects Stellaria aquatica

Archives of Virology - Tập 168 - Trang 1-4 - 2023
Workitu Firomsa Gudeta1,2,3, Ah-Young Shin1, Se Eun Kim1, Kwon Jeong-A1,2, Kwon Seok-Yoon1,2, Jae Sun Moon1,2
1Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea
2Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, Republic of Korea
3Ethiopian Institute of Agricultural Research (EIAR), Holeta, Ethiopia

Tóm tắt

The complete genome sequence of Stellaria aquatica virus B (StAVB), a new member of the genus Polerovirus that infects Stellaria aquatica, was determined using high-throughput RNA sequencing with confirmation by Sanger sequencing. The complete StAVB genome (GenBank accession no. OP389993) is 5,900 nucleotide (nt) long with seven open reading frames (ORF0–5 and ORF3a) that encode putative proteins (P0–P5 and P3a) in a similar configuration to that of other typical poleroviruses. Pairwise sequence comparisons with other poleroviruses showed 38–50% nt sequence identity in the complete genome and 13–24%, 36–45%, 7–68%, and 6–50% amino acid sequence identity in (aa), for the P0, P1–2, P3, and P4 protein, respectively. These data, together with the results of phylogenetic analysis, indicate that StAVB should be classified as a new member of the genus Polerovirus, family Solemoviridae.

Tài liệu tham khảo

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