Complete genome sequence of Staphylococcus aureus, strain ILRI_Eymole1/1, isolated from a Kenyan dromedary camel

Standards in Genomic Sciences - Tập 10 Số 1
Saima Zubair1, Anne Fischer2,3, Anne Liljander3, Jochen Meens4, Jan Hegerman5,6,7, Hadrien Gourlé1, Richard P. Bishop3, Ina Roebbelen3, Mario Younan8, Mudassir Imran Mustafa9, Muhammad Mushtaq1, Erik Bongcam‐Rudloff1, Joerg Jores3
1Department of Animal Breeding and Genetics, SLU Global Bioinformatics Centre, Swedish University of Agricultural Sciences, SE-75007 Uppsala, Sweden
2International Center for Insect Physiology and Ecology, PO Box 30722, Nairobi, Kenya.
3International Livestock Research Institute, PO Box 30709, Nairobi, Kenya
4Department of Infectious Diseases, Institute for Microbiology, University of Veterinary Medicine Hannover, Hannover, Germany
5Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL), Hannover, Germany
6Institute of Functional and Applied Anatomy, Hannover Medical School, Hannover, Germany
7REBIRTH Cluster of Excellence, Hannover, Germany
8Vétérinaires sans Frontières Germany, Nairobi, Kenya.
9Department of Public Health and Caring Science, Uppsala University, 751 22 Uppsala, Sweden.

Tóm tắt

AbstractWe report the genome of a Staphylococcus aureus strain (ILRI_Eymole1/1) isolated from a nasal swab of a dromedary camel (Camelus dromedarius) in North Kenya. The complete genome sequence of this strain consists of a circular chromosome of 2,874,302 bp with a GC-content of 32.88 %. In silico annotation predicted 2755 protein-encoding genes and 76 non-coding genes. This isolate belongs to MLST sequence type 30 (ST30). Phylogenetic analysis based on a subset of 283 core genes revealed that it falls within the human clonal complex 30 (CC30) S. aureus isolate cluster but is genetically distinct. About 79 % of the protein encoding genes are part of the CC30 core genome (genes common to all CC30 S. aureus isolates), ~18 % were within the variable genome (shared among multiple but not all isolates) and ~ 3 % were found only in the genome of the camel isolate. Among the 85 isolate-specific genes, 79 were located within putative phages and pathogenicity islands. Protein encoding genes associated with bacterial adhesion, and secretory proteins that are essential components of the type VII secretion system were also identified. The complete genome sequence of S. aureus strain ILRI_Eymole1/1 has been deposited in the European Nucleotide Archive under the accession no LN626917.1.

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