Complementation of a <i>Treponema denticola flgE</i> Mutant with a Novel Coumermycin A1-Resistant <i>T. denticola</i> Shuttle Vector System

Infection and Immunity - Tập 70 Số 4 - Trang 2233-2237 - 2002
Bo Chi1, Ronald J. Limberger2, Howard K. Kuramitsu3
1Department of Oral Biology, State University of New York at Buffalo, Buffalo, New York 14214, USA.
2David Axelrod Institute of Public Health, New York State Department of Health, Albany, New York 12201
3Department of Oral Biology, State University of New York at Buffalo, Buffalo, New York, 14214

Tóm tắt

ABSTRACT By using the mutated gyrB gene from a spontaneous coumermycin A1-resistant Treponema denticola , an Escherichia coli-T. denticola shuttle vector that renders T. denticola resistant to coumermycin was constructed. The complete T. denticola flgE gene was cloned into the shuttle vector pKMCou, and the vector was transformed into the T. denticola ATCC 33520 flgE erythromycin-resistant knockout mutant HL210. The coumermycin-resistant transformants were motile and restored FlgE activity. This complementation system should prove useful in studying the virulence factors of T. denticola and uncultivable pathogenic spirochetes.

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Thông tin
Thông tin xuất bản

Infection and Immunity

Tập 70 Số 4

2233-2237

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